维生素D对自发性糖尿病大鼠肠道菌群结构和多样性的影响  

Effect of vitamin D on the structure and diversity of intestinal flora in spontaneously diabetic rats

作　　者：陈琳 谭洪兴[1] 郝丽萍[2] 李艳艳 CHEN Lin;TAN Hongxing;HAO Liping;LI Yanyan(Shenzhen Center for Chronic Disease Control,Shenzhen 518020,China;Department of Nutrition and Food Hygiene,School of Public Health,Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430030)

机构地区：[1]深圳市慢性病防治中心,广东深圳518020 [2]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030

出　　处：《中国比较医学杂志》2024年第2期55-62,共8页Chinese Journal of Comparative Medicine

基　　金：深圳医学重点学科建设经费资助(SZXK065);国家自然科学基金(81703226)。

摘　　要：目的探讨维生素D(VD)对自发性糖尿病大鼠肠道菌群的影响。方法自发性肥胖型2型糖尿病(ZDF)大鼠随机分为正常对照(Con)组、VD对照(VD)组、模型(T2DM)组和VD干预(VD+T2DM)组。检测各组大鼠空腹血糖情况和口服糖耐量水平。采用16S rDNA测序技术检测大鼠肠道菌群变化,进行OTU分析(韦恩图)、α多样性分析(chao1、observed species、PD whole tree、shannon和simpson)、β多样性分析(主坐标分析(PCoA))、菌群结构及菌群物种差异性分析(线性判别分析及影响因子(LEfSe)分析)。结果VD干预显著改善了T2DM大鼠的空腹血糖水平和胰岛素抵抗情况(P<0.05)。α多样性结果表明T2DM组和VD+T2DM组的chao1、observed species、PD whole tree、shannon和simpson指数无显著差异(P>0.05);β多样性分析结果显示,与T2DM组相比,VD+T2DM组与Con组的物种相似度更高。各组大鼠肠道菌群的优势菌具有显著差异;相较于T2DM组,VD+T2DM组的Bacteroidetes丰度下降、Firmicutes和Clostridium XIVa丰度增加。结论VD可改善T2DM大鼠的空腹血糖升高和胰岛素抵抗情况;VD可改善T2DM大鼠的肠道菌群结构,降低Bacteroidetes丰度,升高Firmicutes和Clostridium XIVa丰度。Objective To investigate the effect of vitamin D(VD)on intestinal flora in spontaneously diabetic rats.Methods Zucker diabetic fatty rats(ZDF rats)were randomised to control(Con)group,VD control(VD)group,model(T2DM)group,and VD intervention(VD+T2DM)group.Fasting blood glucose profiles and oral glucose tolerance levels were determined in rats of each group.16S rDNA sequencing was used to assess changes in rat intestinal flora.OTU analysis(Venn diagram),αdiversity analysis(chao1,observed species,PD whole tree,and shannon and simpson),βdiversity analysis(principal coordinate analysis(PCoA)),flora structure,and colony species variability analysis(linear discriminant analysis and influence factor(LEfSe)analysis)were also performed.Results VD intervention significantly improved fasting blood glucose levels and insulin resistance in T2DM rats(P<0.05).αdiversity showed no significant differences in chao1,observed species,PD whole tree,and shannon and simpson indices between T2DM and VD+T2DM groups(P>0.05).βdiversity analysis showed that the VD+T2DM group had more species similarity to the Con group than the T2DM group.The dominant bacteria of rat intestinal flora in each group were significantly different.In comparison to the T2DM group,the VD+T2DM group showed a decrease in abundance of Bacteroidetes and increases in abundances of Firmicutes and Clostridium XIVa.Conclusions VD improves fasting glucose elevation and insulin resistance in T2DM rats.VD improves the structure of intestinal flora,decreases Bacteroidetes,and elevates Firmicutes and Clostridium XIVa abundances in T2DM rats.

关 键 词：维生素D 糖尿病 肠道菌群 16S rDNA测序 血糖 胰岛素抵抗 

分 类 号：R-33[医药卫生]