实验用鹌鹑遗传检测方法的初步建立  

Establishment of a genetic monitoring method for laboratory quails

作　　者：何洋 章秀林 张秋宇 张晓璐 傅博 王文栋 杜小燕[2] 陈振文[2] 李长龙[2] HE Yang;ZHANG Xiulin;ZHANG Qiuyu;ZHANG Xiaolu;FU Bo;WANG Wendong;DU Xiaoyan;CHEN Zhenwen;LI Changlong(School of Nursing,Dalian Medical University,Dalian 116044,China;Capital Medical University School of Basic Medical Sciences,Beijing 100069;Fuwai Hospital,Chinese Academy of Medical Sciences,Beijing 100037)

机构地区：[1]大连医科大学护理学院,辽宁大连116044 [2]首都医科大学基础医学院,北京100069 [3]中国医学科学院阜外医院,北京100037

出　　处：《中国比较医学杂志》2024年第2期101-107,共7页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划(2022YFF0711005,2021YFF0703200);国家自然科学基金(32070537);北京市自然科学基金-北京市教育委员会重点项目(KZ20210025037)。

摘　　要：目的建立封闭群实验用鹌鹑微卫星DNA遗传检测方法。方法通过文献检索,筛选鹌鹑微卫星位点,利用种间转移法,在鹌鹑近缘物种—鸡和鸭筛选适合鹌鹑的微卫星DNA位点。提取鹌鹑肝组织DNA作为模板,并通过PCR扩增以及琼脂糖凝胶电泳技术,对相应的位点进行筛选。最后根据所选微卫星位点扩增情况、等位基因数目和多态性等,筛选出适用于鹌鹑遗传质量检测的微卫星位点组合并建立检测方法。结果初步确定适用于封闭群实验用鹌鹑遗传检测的23个微卫星位点组合。结论初步建立了实验用鹌鹑的遗传质量检测方法。Objective To establish a genetic monitoring method for laboratory quails.Methods Quail microsatellite loci were searched in the literature,and microsatellite DNA loci suitable for quails were screened by an interspecific transfer method in closely related species,namely chickens and ducks.Quail liver DNA was extracted as a template,and the corresponding loci were screened by PCR amplification and agarose gel electrophoresis.On the basis of amplification of the selected microsatellite loci,the number of alleles,polymorphisms,and microsatellite loci combinations for quail genetic quality detection were selected and detection method were developed.Results We preliminary determined 23 microsatellite loci for genetic monitoring of closed⁃colony laboratory quails.Conclusions A genetic monitoring method for laboratory quails was preliminary developed.

关 键 词：实验用鹌鹑 遗传检测方法 微卫星 封闭群 

分 类 号：R-33[医药卫生]