SPP1、DEC1、C1QTNF6蛋白与口腔鳞状细胞癌患者临床病理指标及预后的关系  

Relationships between SPP1,DEC1,C1QTNF6 and clinicopathologic outcomes of oral squamous cell carcinomas

作　　者：付勇青 徐三会 赵岩 王丽丽 FU Yongqing;XU Sanhui;ZHAO Yan;WANG Lili(Laboratory Department of Xingtai People's Hospital,Xingtai 054001,Hebei,China)

机构地区：[1]邢台市人民医院检验科,河北邢台054001

出　　处：《癌变．畸变．突变》2024年第2期107-111,117,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：邢台市科技计划项目(2022ZC097)。

摘　　要：目的:探讨口腔鳞状细胞癌患者血清重组人分泌型磷蛋白1(SPP1)、软骨分化的表达基因1(DEC1)和补体C1q/肿瘤坏死因子相关蛋白6(C1QTNF6)的表达水平与其临床病理指标及预后的关系。方法:免疫组织化学染色法和电化学发光免疫分析法检测88例口腔鳞状细胞癌患者癌组织和血清中SPP1、DEC1和C1QTNF6蛋白的表达水平;Pearson相关性分析和Kaplan-Meier生存分析法分析患者血清SPP1、DEC1和C1QTNF6蛋白表达水平与其临床病理指标的相关性和对患者预后的影响。多因素Cox回归法分析影响口腔鳞状细胞癌患者预后的危险因素。结果:免疫组织化学染色结果显示口腔鳞状细胞癌患者癌组织中SPP1、DEC1和C1QTNF6蛋白的阳性表达率较癌旁组织分别增加了1.94、2.98和2.35倍(P<0.05或P<0.01);电化学发光免疫分析法结果显示口腔鳞状细胞癌患者血清SPP1、DEC1和C1QTNF6蛋白表达水平较正常人分别上调了8.61、6.20和4.03倍(P<0.05或P<0.01);Pearson相关性分析结果显示患者血清SPP1、DEC1和C1QTNF6蛋白表达水平与患者发生多灶嗜神经侵犯、肿瘤浸润程度、淋巴结转移、较高的TNM分期呈正相关(P<0.05);Kaplan-Meier生存分析结果显示,血清SPP1、DEC1和C1QTNF6蛋白高表达水平的口腔鳞状细胞癌患者总生存率低;多因素Cox回归分析结果表明多灶嗜神经侵犯、肿瘤高浸润度、淋巴结转移和高的TNM分期是影响预后的危险因素(P<0.05)。结论:口腔鳞状细胞癌患者癌组织和血清中SPP1、DEC1和C1QTNF6蛋白表达水平升高,且与患者发生多灶嗜神经侵犯、肿瘤浸润和淋巴结转移、较高的TNM分期呈正相关,而与患者预后呈负相关。OBJECTIVE:To investigate correlations of serum secreted phosphoprotein 1(SPP1),differentiated embryo-chonrocyte expressed gene 1(DEC1)and the complement C1q/tumor necrosis factor associated protein 6(C1QTNF6)levels with clinicopathologic outcomes of oral squamous cell carcinomas.METHODS:Immunohistochemical staining and electrochemiluminescence immunoassay were used to detect protein expression levels of SPP1,DEC1 and C1QTNF6 in tumor tissues and serum samples from 88 patients with oral squamous cell carcinoma.Pearson correlation and Kaplan-Meier survival analyses were used to analyze correlations between protein expression levels of serum SPP1,DEC1 and C1QTNF6 and clinicopathological outcomes.Multivariate Cox regression analysis was used to analyze the risk factors affecting the prognosis of patients with oral squamous cell carcinoma.RESULTS:Results from immunohistochemical staining showed that positive expression levels of SPP1,DEC1 and C1QTNF6 proteins in carcinoma tissues were 1.94,2.98 and 2.35 times higher than those in adjacent normal tissues(P<0.05 or P<0.01).Results from the electrochemiluminescence immunoassay showed that expression levels of serum SPP1,DEC1 and C1QTNF6 in patients were up-regulated by 8.61,6.20 and 4.03 times(P<0.05 or P<0.01)compared with normal controls.Pearson correlation analysis results showed that expression levels of serum SPP1,DEC1 and C1QTNF6 were positively correlated with the occurrence of multifocal neurotropic invasion,degree of tumor invasion,lymph node metastasis,and higher TNM stage(P<0.05).Kaplan-Meier survival analysis showed that patients with high serum SPP1,DEC1 and C1QTNF6 protein expression levels had a low overall survival rate.Multivariate Cox regression analysis showed that multifocal neurotropic invasion,high tumor invasion,lymph node metastasis and high TNM stage were the risk factors for prognosis of patients(P<0.05).CONCLUSION:Abnormally elevated serum SPP1,DEC1 and C1QTNF6 proteins in patients were positively correlated with multifocal perineural invasion,

关 键 词：口腔鳞状细胞癌 重组人分泌型磷蛋白1 软骨分化的表达基因1 补体C1q/肿瘤坏死因子相关蛋白6 预后 

分 类 号：R730.2[医药卫生—肿瘤]