miR-544a在结直肠癌组织中的表达及其意义  

Expression and significance of miR-544a in patients with colorectal cancer

作　　者：童晶 王丹[2] TONG Jing;WANG Dan(Pathology Department of Yangzhou Jiangdu People's Hospital,Yangzhou 225299,Jiangsu;Pathology Department of Shangqiu First People's Hospital,Shangqiu 476005,Henan,China)

机构地区：[1]扬州市江都人民医院病理科,江苏扬州225299 [2]商丘市第一人民医院病理科,河南商丘476005

出　　处：《癌变．畸变．突变》2024年第2期124-128,137,共6页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨结直肠癌患者组织中微小RNA-544a(miR-544a)的表达水平及其与患者预后的关系。方法:选取2016年1月—2019年2月住院并接受手术的150例结直肠癌患者,采用实时荧光定量PCR法检测癌组织中miR-544a的表达情况,免疫组织化学法检测癌组织中Ki67、CD4、CD8表达情况。Mann-Whitney U检验、卡方检验和Fisher精确检验分析癌组织中miR-544a表达水平与患者临床病理指标的关系。单因素和多因素Cox回归分析影响结直肠癌预后的因素。Kaplan-Meier法分析结直肠癌组织中miR-544a表达水平与患者无病生存期(DFS)、总生存期(OS)的关系。结果:结直肠癌组织中miR-544a相对表达水平为1.85±0.15,高于癌旁正常组织的1.06±0.21,差异具有统计学意义(P<0.01)。miR-544a表达水平与周围神经侵犯率、T分期、N分期有关(均为P<0.01),miR-544a高表达组神经侵犯率高,T、N分期较晚。免疫组织化学检测结果显示,miR-544a高表达组Ki67评分为120.2±33.7,低表达组Ki67评分为112.4±40.8,两组差异无统计学意义(P>0.05);miR-544a高表达组患者CD4、CD8评分分别为15.5±5.3和24.8±7.6,均低于miR-544a低表达组(分别为21.3±7.2和29.6±8.9),差异均具有统计学意义(均为P<0.05)。单因素、多因素Cox回归分析显示肿瘤N、M分期及miR-544a表达水平与CRC患者根治术后DFS、OS显著相关(均为P<0.01)。结论:结直肠癌组织中miR-544a表达水平上调,miR-544a高表达与结直肠癌患者较晚的N、M分期以及不良预后有关,且miR-544a可能是反映免疫治疗效果的潜在标志物。OBJECTIVE:To explore expression level and clinical prognostic value of microRNA-544a(miR-544a)in tissues of colorectal cancer patients.METHODS:From January 2016 to February 2019,150 patients with colorectal cancer were selected.Real time fluorescence quantitative PCR was used to detect expression levels of miR-544a in tissues,and immunohistochemistry was used to detect the expression of Ki67,CD4,and CD8 in tissues.Mann Whitney U-test,chi square test,and Fisher′s exact test were used to analyze relationships between miR-544a expression level in cancer tissue and clinical pathological indicators of patients.Univariate and multivariate Cox regressions were used to analyze factors affecting the prognosis of colorectal cancer.The Kaplan-Meier method was used to analyze relationships between expression levels of miR-544a in tissues and disease-free survival(DFS)and overall survival(OS)of patients.RESULTS:The relative expression level of miR-544a in colorectal cancer tissue(1.85±0.15)was significantly higher than that in the adjacent normal tissues(1.06±0.21)(P<0.01).The expression level of miR-544a was related to the incidence of peripheral nerve invasion,T staging,and N staging(all P<0.01),while the high expression group of miR-544a had a higher incidence of nerve invasion and a later T and N staging.The immunohistochemical results showed that the Ki67 score of the miR-544a high expression group was 120.2±33.7,while the Ki67 score of the low expression group was 112.4±40.8,with no significant difference between the two groups(P>0.05).The CD4 and CD8 scores of patients in the miR-544a high expression group were 15.5±5.3 and 24.8±7.6,respectively,lower than those in the miR-544a low expression group(21.3±7.2 and 29.6±8.9,respectively),with statistically significant differences(all P<0.05).Univariate and multivariate COX regression showed significant correlations between tumor N,M staging,and miR-544a expression levels and postoperative DFS and OS in CRC patients(all P<0.01).CONCLUSION:Upregulation of miR-544a

关 键 词：结直肠癌 微小RNA miR-544a 预后 肿瘤标志物 

分 类 号：R735.3[医药卫生—肿瘤]