青钱柳苦荞膳食补充剂对大鼠的28天经口毒性试验  

Subacute toxicity of a dietary supplement containing Cyclocarya paliurus and Fagopyrum tataricum in rats

作　　者：周庆红[1] 何宁[1] 张书婧[1] 刘洪波 魏婕敏 钱智勇[1] ZHOU Qinghong;HE Ning;ZHANG Shujing;LIU Hongbo;WEI Jiemin;QIAN Zhiyong(Department of Toxicology,Tianjin Centers for Disease Control and Prevention,Tianjin 300011;Department of Occupational and Environmental Health/School of Public Health/Tianjin Key Laboratory of Environment,Nutrition and Public Health/Center for International Collaborative Research on Environment,Nutrition and Public Health,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津市疾病预防控制中心毒理室,天津300011 [2]天津医科大学公共卫生学院劳动与环境卫生学系/天津市环境营养与人群健康重点实验室/环境营养与人群健康国际联合研究中心,天津300070

出　　处：《癌变．畸变．突变》2024年第2期133-137,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(82173485);天津市应用基础研究多元投入青年项目(21JCQNJC01810)。

摘　　要：目的:研究青钱柳苦荞膳食补充剂对大鼠亚急性毒性。方法:将Wistar大鼠按体质量随机分为4组,即青钱柳苦荞膳食补充剂3个剂量组(1.67、3.33、6.67 g/kg)和1个常规基础饲料对照组。分别喂饲相应饲料28 d,自由进食饮水,观察大鼠体质量、食物利用率、血常规、血生化、尿液指标,大鼠麻醉后解剖进行大体观察、计算主要脏器的脏器系数并进行组织病理学检查。结果:实验组大鼠的体质量增长正常,未见明显毒性反应,其血常规、血生化、尿液指标、脏器系数与对照组比较差异均无统计学意义(均为P>0.05)。大鼠解剖时大体观察未发现明显异常。个别实验组大鼠心、肾脏质量与对照组比较差异具有统计学意义(P<0.05),病理组织学检查未发现青钱柳苦荞膳食补充剂引起的典型病理组织学改变和特异性损伤改变。结论:在本实验条件下,该青钱柳苦荞膳食补充剂经大鼠28天经口毒性试验未见明显的毒性作用。OBJECTIVE:To investigate the subacute toxicity of a dietary supplement containing Cyclocarya paliurus and Fagopyrum tataricum in rats.METHODS:Wistar rats were randomly divided into four groups according to body weight,including three dosage groups of the dietary supplement(1.67,3.33 and 6.67 g/kg)and one control group fed with a conventional basal diet.The corresponding feed was given for 28 days with free access to water.Body weight,food utilization rate,blood routine,blood biochemistry,urine parameters,organ coefficients and histopathological examination of the major organs were observed after anesthesia and dissection.RESULTS:The experimental rats had normal body weight growth and no obvious toxic reactions were observed.There was no statistical difference in hematological,biochemical,urine parameters and organ coefficients between the experimental and the control groups(P>0.05).No abnormality was found in gross observation during animal dissection.Some experimental groups showed statistically significant differences(P<0.05)in heart and kidney weight compared with the control group.Histopathological examination revealed no typical pathological histological changes or specific damage caused by the dietary supplement containing Cyclocarya paliurus and Fagopyrum tataricum.CONCLUSION:Under the conditions of this experiment,the dietary supplement containing Cyclocarya paliurus and Fagopyrum tataricum did not induce obvious toxicity in rats after oral exposure for 28 days.

关 键 词：青钱柳 苦荞 亚急性毒性 WISTAR大鼠 膳食补充剂 

分 类 号：R114[医药卫生—卫生毒理学]