金莲花饲用安全性评价  

Evaluation of feeding safety of Trollius chinensis

作　　者：崔莹 马德志 于侃超 李慧 张可勇 刘吉成 CUI Ying;MA De-zhi;YU Kan-chao;LI Hui;ZHANG Ke-yong;LIU Ji-cheng

机构地区：[1]齐齐哈尔医学院科研处黄芪产业研究院,黑龙江齐齐哈尔161006

出　　处：《饲料研究》2024年第4期92-98,共7页Feed Research

基　　金：黑龙江省应用技术研究与开发计划项目(项目编号:GA19C009-03)。

摘　　要：试验旨在探究金莲花饲用安全性,对金莲花进行急性毒性试验、遗传毒性试验(细菌回复突变试验、哺乳动物红细胞微核试验、小鼠精母细胞染色体畸变)和亚慢性毒性试验。结果显示,昆明小鼠半数致死量(LD50)大于10.0 g/kg·BW。遗传毒性试验结果未见金莲花对试验动物致突变作用。大鼠致畸试验结果显示,各剂量组孕鼠未出现中毒体征,未观察到大鼠的生殖毒性,未见致畸作用。亚慢性毒性试验未见大鼠出现中毒表现以及死亡发生,各剂量组大鼠的体重、增重、进食量、食物利用率、脏体比与阴性对照相比差异均不显著(P>0.05)。各组大鼠的血常规、血液生化、尿常规等指标均在正常范围内,心脏、肝脏、胰脏、肺、肾脏、脑、胃、睾丸和子宫等脏器的色泽、大小和形态结构均未见明显异常。研究表明,金莲花毒性小,饲用安全性较高。The purpose of the experiment was to explore the feeding safety of Trollius chinensis.Acute toxicity test,genotoxicity test(bacterial reversion mutation test,mammalian erythrocyte micronucleus test,mouse spermatocyte chromosome aberration),and subchronic toxicity test were conducted on Trolliuschinensis.The results showed that the median lethal dose(LD50)of Kunming mice was more than 10.0 g/kg·BW.The results of the genotoxicity test showed no mutagenic effect of Trolliuschinensison experimental animals.The results of the rat teratogenicity test showed that there were no toxic signs,no reproductive toxicity,and no teratogenicity in pregnant rats of each dose group.The subchronic toxicity test showed no toxicity and death in rats.There was no significant difference in body weight,weight gain,food intake,food utilization rate,and viscera-body ratio between rats in each dose group and negative control group(P>0.05).The blood routine,blood biochemistry,urine routine,and other indexes of rats in each group were all in the normal range,and the color,size,morphology,and structure of the main organs such as heart,liver,pancreas,lung,kidney,brain,stomach,testis,and uterus were not obviously abnormal.The study indicates that Trolliuschinensis has low toxicity and high feeding safety.

关 键 词：金莲花 遗传毒性试验 急性毒性试验 亚慢性毒性试验 安全性评价 

分 类 号：S816.7[农业科学—饲料科学]