高效液相色谱法测定氟苯尼考原料药  

Determination of Flufenicol API by High Performance Liquid Chromatography

作　　者：郭芳芳[1] 戴超 狄大铃 王果 Guo Fangfang;Dai Chao;Di Daling;Wang Guo(The Physical and Chemical Testing Center of Jiangsu Province,Nanjing Jiangsu 210042,China;Nanjing Tianzong Yikang Biotechnology Co.,Ltd.,Nanjing Jiangsu 210042,China;Jiangsu Jinshiyuan Liquor Industry Co.,Ltd.,Nanjing Jiangsu 210042,China;Productivity Center of Jiangsu Province,Nanjing Jiangsu 210042,China)

机构地区：[1]江苏省理化测试中心,江苏南京210042 [2]南京天纵易康生物科技股份有限公司,江苏南京210042 [3]江苏今世缘酒业股份有限公司,江苏南京210042 [4]江苏省生产力促进中心,江苏南京210042

出　　处：《山西化工》2024年第3期37-39,共3页Shanxi Chemical Industry

基　　金：江苏省生产力促进中心青年人才基金项目(H2022-4605-jspc-21)。

摘　　要：本研究建立了氟苯尼考药的液相色谱测定方法。通过研究色谱柱分离、定性波长、流动相选择等条件确定检测方法。并通过检出限、定量限、回收率和重复性实验评价方法。结果表明,选择TC-C18柱作为分离柱,最佳波长为223 nm,最佳流动相为25%乙腈水溶液,氟苯尼考线性达到0.99975,检出限为0.05μg/mL,定量限为0.15μg/mL,添加量为0.5~3μg时回收率达到88.7%~100%,重复性RSD达到0.64%。此方法高效准确,能为氟苯尼考药主含量测定提供参考,也能为其他兽药氟苯尼考的测定提供思路。This study established a liquid chromatography method for the determination of flufenicol.Determine the detection method by studying conditions such as chromatographic column separation,qualitative wavelength,and mobile phase selection.And evaluate the method through detection limit,quantification limit,recovery rate,and repeatability experiments.The results showed that TC-C18 column was selected as the separation column,with the optimal wavelength of 223 nm and the optimal mobile phase of 25%acetonitrile aqueous solution.The linearity of fluorophenicol reached 0.99975,and the detection limit was 0.05μg/mL,with a quantification limit of 0.15μg/mL,with an addition amount of 0.5-3μg.The recovery rate at g reaches 88.7%~100%,and the repeatability RSD reaches 0.64%.This method is efficient and accurate,and can provide reference for the determination of the main content of flufenicol,as well as ideas for the determination of other veterinary drugs such as flufenicol.

关 键 词：高效液相色谱 氟苯尼考 原料药 主含量 

分 类 号：O657.72[理学—分析化学]