储藏小麦中草酸青霉的分离鉴定及其侵染防控  

Isolation and identification of Penicillium oxalicum from stored wheat and its infection prevention and control

作　　者：张双双 陈亮[1] 许茜茜 孙云祥 薛战英 ZHANG Shuang-shuang;CHEN Liang;XU Xi-xi;SUN Yun-xiang;XUE Zhan-ying(College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,Henan,China)

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《粮食与油脂》2024年第4期49-53,79,共6页Cereals & Oils

基　　金：国家自然科学基金项目(32172280);国家标准制修订项目(20212911-T-449)。

摘　　要：采用梯度稀释法从不同来源小麦中分离青霉菌株;采用经典形态学分类鉴定方法和ITS序列比对鉴定青霉菌株种属;采用琼脂稀释平板法测定香茅醇对青霉菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MFC);采用熏蒸法考察香茅醇对青霉侵染小麦的防控效果。结果发现:草酸青霉是储藏小麦中的优势青霉;香茅醇对草酸青霉菌丝生长的MIC、MFC均为0.1250μL/mL;当香茅醇含量为0.250μL/mL时,草酸青霉侵染的小麦储藏5 d均未发生霉变,表明香茅醇对青霉侵染小麦具有良好的防控效果。Penicillium strains were isolated from wheat from different sources by gradient dilution method.The isolated strains were identified by classical classification method and ITS sequence comparison.Minimal inhibitory concentration(MIC)and minimal fungicidal concentration(MFC)of citronellol against Penicillium strains were determined by agar dilution method,and the prevention effect of citronellol on the infection of wheat by Penicilium strains were investigated by fumigationmethod.The results showed that Penicillium oxalicum was the dominant Penicillium in stored wheat.The MIC and MFC of citronellol against Penicillium oxalicum was 0.1250μL/mL.When the content of citronellol was 0.250μL/mL,the wheat infected by Penicillium oxalicum did not become mildew after 5 d of storage,indicating that citronellol had a good control effect on Penicillium infected wheat.

关 键 词：小麦 粮食储藏 草酸青霉 分离鉴定 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]