基于LIS系统数据建立广西南宁地区0-12岁健康儿童血清碱性磷酸酶参考区间  

The Development of Reference Intervals of Serum Alkaline Phosphatase for Healthy Children Aged 0 to 12 Years in Nanning,Guangxi Based on a Laboratory Information System

作　　者：易莉玲 韦尚谋 朱春玲[1] 韦玉红 周冬谊 彭契六 YI Liling;WEI Shangmou;ZHU Chunling;WEI Yuhong;ZHOU Dongyi;PENG Qiliu(Department of Clinical Laboratory,International Zhuang Medicine Hospital Affiliated toGuangxi University of Traditional Chinese Medicine,Nanning 530201,China)

机构地区：[1]广西中医药大学附属国际壮医医院检验科,广西南宁530201

出　　处：《标记免疫分析与临床》2024年第1期136-141,共6页Labeled Immunoassays and Clinical Medicine

基　　金：广西国际壮医医院引进人才科研启动基金项目(编号:RCYJ202201);广西国际壮医医院优势学科项目(临床检验诊断学)(编号:院字[2021]33号);广西国际壮医医院"青苗工程"培育项目(编号:院字[2022]203)。

摘　　要：目的 利用实验室信息系统(LIS)数据,建立广西南宁地区0~12岁健康儿童血清碱性磷酸酶(ALP)的参考区间,为临床诊疗工作提供可靠依据。方法 筛选广西中医药大学附属国际壮医医院0~12岁健康儿童共2 314例为参考人群,另筛选240例为参考区间验证组。将研究对象分为<28d、28d至<1岁、1岁至<3岁、3岁至<6岁、6岁至<9岁、9岁至<12岁6个年龄组。使用四分位间距法剔除离群值后,采用Kolmogorov-Smirnov Z检验分析数据分布特性,通过Mann-Whitney U检验分析ALP水平在性别、相邻年龄组间的差异,Spearman秩相关分析ALP水平与年龄的相关性。采用非参数法(P2.5~P97.5)建立血清ALP参考区间。结果 剔除离群值、重复值后,共2 256例纳入研究,数据呈偏态分布。ALP水平在性别间差异无统计学意义(P>0.05)、与年龄呈正相关(r=0.526,P<0.05)。将差异无统计学意义的年龄组合并,最终建立血清ALP的参考区间:105.98~288.03U/L(<28d)、136.60~350.40U/L(28d至<3岁)、153.00~354.10U/L(3岁至<12岁),并通过验证。建立的参考区间与试剂说明书、行业标准提供的参考区间相比均有一定的差异。结论 本研究建立了0~12岁健康儿童血清ALP参考区间,对于提高儿童血清ALP检测在本地区临床实验室的应用价值具有重要意义。Objective To investigate the distribution of the dataset of serum alkaline phosphatase(ALP)from Laboratory Information System(LIS)and to establish the reference intervals of serum ALP for healthy children aged 0-12 years in Nanning,Guangxi,so as to provide a reliable reference basis for clinical diagnosis and treatment.Methods A total of 2314 healthy children aged 0 to 12 years in Nanning area who visited the International Zhuang Medicine Hospital Affiliated to Guangxi University of Traditional Chinese Medicine for physical examination were selected as the reference population,while another 240 cases were collected for the reference interval validation group.All study subjects were divided into 6 age groups:<28d,28 d to<1 year,1 year to<3 years,3 years to<6 years,6 years to<9 years,and 9 years to<12 years.Kolmogorov-Smirnov Z test was used to analyze the distribution of the dataset after removing outliers using the interquartile range method.The Mann-Whitney U test was used to analyze the differences in ALP levels between sex and adjacent age groups,an Spearman’s rank correlation was used to investigate the correlation between ALP levels and age groups.The non-parametric method(P 2.5 to P 97.5)was used to establish serum ALP reference intervals.Results After excluding outliers and duplicates,a total of 2256 cases were included in the study cohort,and the dataset showed a skewed distribution.There was no statistically significant difference in ALP levels between sexes(P>0.05),while a positive correlation between ALP levels and age(r=0.526,P<0.05)was observed.Age groups with no statistically significant differences were combined,and the reference intervals of serum ALP which had been verified were 105.98-288.03 U/L(<28 d),136.60-350.40 U/L(28 d to<3 years),and 153.00-354.10 U/L(3 years to<12 years),respectively.There were certain differences compared with the reference intervals provided in the instructions and industry standards.Conclusion The current study establishs reference intervals for serum ALP in healthy

关 键 词：参考区间 健康儿童 碱性磷酸酶 实验室信息系统 

分 类 号：R-331[医药卫生]