Systematic identification of wheat spike developmental regulators by integrated multiomics, transcriptional network, GWAS, and genetic analyses  

作　　者：Xuelei Lin Yongxin Xu Dongzhi Wang Yiman Yang Xiaoyu Zhang Xiaomin Bie Lixuan Gui Zhongxu Chen Yiliang Ding Long Mao Xueyong Zhang Fei Lu Xiansheng Zhang Cristobal Uauyo Xiangdong Fu Jun Xiao 

机构地区：[1]Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [4]Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Taian,Shandong 271018,China [5]Department of Life Science,Tcuni Inc.,Chengdu,Sichuan 610000,China [6]John Innes Centre,Norwich Research Park,Norwich NR47UH,UK [7]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [8]CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),Institute of Genetics and Developmental Biology,CAS,Beijing 100101,China

出　　处：《Molecular Plant》2024年第3期438-459,共22页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China(31921005);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010204);the National Key Research and Development Program of China(2021YFD1201500);the Major Basic Research Program of Shandong Natural Science Foundation of China(ZR2019ZD15).

摘　　要：The spike architecture of wheat plays a crucial role in determining grain number,making it a key trait for optimization in wheat breeding programs.In this study,we used a multi-omic approach to analyze the transcriptome and epigenome profiles of the young spike at eight developmental stages,revealing co-ordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering transition.We constructed a core transcriptional regulatory network(TRN)that drives wheat spike formation and experimentally validated a multi-layer regulatorymodule involving TaSPL15,TaAGLG1,and TaFUL2.By integrating the TRN with genome-wide association studies,we identified 227 transcription factors,including 42 with known functions and 185 with unknown functions.Further investigation of 61 novel transcription factors using multiple homozygous mutant lines revealed 36 transcription factors that regulate spike architecture or flowering time,such as TaMYC2-A1,TaMYB30-A1,and TaWRKY37-A1.Of particular interest,TaMYB30-A1,downstream of and repressed by WFzP,was found to regulate fertile spikelet number.Notably,the excellent haplotype of TaMYB30-A1,which contains a C allele at the WFzP binding site,was enriched during wheat breeding improvement in China,leading to improved agronomic traits.Finally,we constructed a free and open access Wheat Spike Multi-Omic Database(http://39.98.48.156:8800/#/).Our study identifies novel and high-confidence regulators and offers an effective strategy for dissecting the genetic basis of wheat spike development,with practical value forwheat breeding.

关 键 词：wheat spike development EPIGENOME TRN TaMYB30-A1 breeding selection 

分 类 号：S512.1[农业科学—作物学]