慢性牙周炎患者龈沟液miR-155、miR-21水平及其临床意义  

Levels and clinical significance of miR-155 and miR-21 in gingival crevicular fluid of patients with chronic periodontitis

作　　者：郭娇娇[1] 卢丽先[1] 巩兰平[1] 张学强[1] 李蕾[1] 刘英奇[1] GUO Jiaojiao;LU Lixian;GONG Lanping;ZHANG Xueqiang;LI Lei;LIU Yingqi(Department of Stomatology,Handan Municipal Central Hospital,Handan,Hebei 056001,China)

机构地区：[1]河北省邯郸市中心医院口腔科,河北邯郸056001

出　　处：《检验医学与临床》2024年第7期889-894,共6页Laboratory Medicine and Clinic

基　　金：河北省卫生健康委员会科研基金项目(20191860)。

摘　　要：目的检测慢性牙周炎(CP)患者龈沟液中微小RNA-155(miR-155)、微小RNA-21(miR-21)表达水平变化及其临床意义。方法选择2021年10月至2022年10月该院142例CP患者作为CP组,根据牙周炎严重程度分为轻度CP组、中度CP组、重度CP组。随访1个月,根据临床疗效分为疗效良好组、疗效较差组。同期142例健康志愿者作为对照组。采用实时荧光定量聚合酶链反应检测龈沟液miR-155、miR-21表达水平。利用Pearson相关分析CP患者龈沟液miR-155、miR-21表达水平与牙龈指数(GI)、牙槽骨吸收程度(ABL)、探诊深度(PD)、附着丧失(AL)的相关性。采用多因素Logistic回归分析CP患者疗效的影响因素,采用受试者工作特征(ROC)曲线评估miR-155、miR-21对CP患者疗效的预测价值。结果CP组miR-155、miR-21表达水平高于对照组(P<0.05)。CP组PD、AL、ABL、GI明显高于对照组(P<0.05)。轻度CP组、中度CP组、重度CP组miR-155、miR-21表达水平依次升高(P<0.05)。疗效较差组miR-155、miR-21表达水平高于疗效良好组(P<0.05)。龈沟液miR-155、miR-21表达水平均与PD、AL、ABL、GI呈正相关(P<0.05)。多因素Logistic回归分析显示,病情严重程度、miR-155、miR-21是CP患者疗效的影响因素(P<0.05)。ROC曲线分析结果显示,miR-155、miR-21预测CP患者疗效较差的曲线下面积(AUC)分别为0.937(95%CI:0.888~0.986)、0.818(95%CI:0.715~0.921),二者联合预测的AUC为0.960(95%CI:0.920~0.998),优于单一指标预测。结论CP患者龈沟液miR-155、miR-21表达水平升高,与牙周炎严重程度、牙周临床指标及临床疗效有关,2项指标或可作为CP患者疗效的潜在预测标志物。Objective To detect the expression levels of microRNA-155(miR-155)and microRNA-21(miR-21)in gingival crevicular fluid of the patients with chronic periodontitis(CP),and to investigate their clinical significance.Methods A total of 142 patients with CP in this hospital from October 2021 to October 2022 were selected as the CP group and divided into the mild CP group,moderate CP group and severe CP group based on the severity of periodontitis.Follow up lasted for 1 month.The patients were divided into the good efficacy group and poor efficacy group according to the clinical efficacy.During the same period,142 healthy volunteers served as the control group(NC group).The real-time fluorescence quantitative polymerase chain reaction method was applied to detect the expression levels of miR-155 and miR-21 in gingival crevicular fluid.The Pearson correlation was applied to analyze the correlation between the expression levels of miR-155 and miR-21 in gingival crevicular fluid with the gingival index(GI),alveolar bone resorption(ABL),probing depth(PD)and attachment loss(AL)in the patients with CP.The multivariate Logistic regression was applied to analyze the influencing factors of efficacy in the patients with CP,the receiver operating characteristic(ROC)curve was applied to evaluate the predictive value of miR-155 and miR-21 for the efficacy in the patients with CP.Results The expression levels of miR-155 and miR-21 in the CP group were higher than those in the NC group(P<0.05).The levels of PD,AL,ABL and GI in the CP group were obviously higher than those in the NC group(P<0.05).The expression levels of miR-155 and miR-21 in the mild CP group,moderate CP group and severe CP group were increased gradually(P<0.05).The expression levels of miR-155 and miR-21 in the poor efficacy group were higher than those in the good efficacy group(P<0.05).The expression levels of miR-155 and miR-21 in gingival crevicular fluid were positively correlated with PD,AL,ABL and GI(P<0.05).The multivariate Logistic regression analysis showed

关 键 词：慢性牙周炎 微小RNA-155 微小RNA-21 疗效 牙龈指数 牙槽骨吸收程度 

分 类 号：R781.4[医药卫生—口腔医学]