circNRIP1对宫颈癌细胞增殖、迁移及侵袭能力的影响研究  

Effect of circNRIP1 on the proliferation,migration,and invasion ability of cervical cancer cells

作　　者：葛琴[1] 蔡平 张玲玲 曹晓莉[1] GE Qin;CAI Ping;ZHANG Lingling;CAO Xiaoli(Department of Clinical Laboratory,Tumor Hospital of Nantong,Nantong,Jiangsu 226000,China)

机构地区：[1]江苏省南通市肿瘤医院检验科,江苏南通226000

出　　处：《国际检验医学杂志》2024年第7期847-852,共6页International Journal of Laboratory Medicine

基　　金：南通市卫生健康委员会科研课题(MSZ2022030)。

摘　　要：目的探讨环状RNA核受体相互作用蛋白1(circNRIP1)在宫颈癌组织中的表达,并初步考察其对宫颈癌细胞增殖、迁移及侵袭能力的影响。方法采用荧光定量PCR(qPCR)检测circNRIP1在宫颈癌组织和癌旁组织中的表达水平,并通过细胞增殖实验(MTS法)、细胞划痕实验和Transwell Matrigel实验分别考察circNRIP1对宫颈癌细胞增殖、迁移及侵袭能力的影响。结果与癌旁组织相比,circNRIP1在宫颈癌组织中的表达显著升高(P<0.001);circNRIP1在HeLa细胞系中表达水平最低,在SiHa细胞系中表达水平最高,与其他细胞系circNRIP1表达水平比较差异均有统计学意义(P<0.001)。在SiHa细胞系中,与NC组比较,shRNA1、shRNA2、shRNA3慢病毒均可显著降低circNRIP1的表达水平,其中shRNA2的敲低能力最高,在HeLa细胞系中,与OV-NC组比较,circNRIP1在OV-cNRIP1组的表达水平更高(P<0.01)。MTS实验结果显示,在HeLa细胞系中,与OV-NC组比较,OV-cNRIP1组的HeLa细胞增殖能力更高(P<0.001);在SiHa细胞系中,与sh-NC组比较,sh-cNRIP1组的SiHa细胞增殖能力更低(P<0.001)。划痕实验结果显示,在SiHa细胞系中,与sh-NC组比较,sh-cNRIP1组的SiHa细胞迁移能力更低(P<0.001);在HeLa细胞系中,与OV-NC组比较,OV-cNRIP1组的HeLa细胞迁移能力显著增强(P<0.001);Transwell Matrigel实验结果显示,在SiHa细胞系中,与sh-NC组比较,sh-cNRIP1组的SiHa细胞穿膜数显著减少(P<0.001);在HeLa细胞系中,与OV-NC组比较,OV-cNRIP1组的HeLa细胞穿膜数显著增多(P<0.001)。结论circNRIP1在宫颈癌组织中的表达上调,circNRIP1能显著促进宫颈癌细胞的增殖、迁移及侵袭。Objective To investigate the expression of circNRIP1 in cervical cancer and its effects on the proliferation,migration and invasion ability of cervical cancer cells.Methods Quantitative fluorescent PCR(qPCR)was used to detect the expression level of circNRIP1 in cervical cancer tissue and adjacent tissues.The effects of circNRIP1 on the proliferation,migration,and invasion ability of cervical cancer cells were investigated by cell proliferation assay(MTS method),cell scratch assay,and Transwell Matrigel assay,respectively.Results Compared with adjacent tissues,the expression of circNRIP1 was significantly increased in cervical cancer tissues(P<0.001).The expression level of circNRIP1 was the lowest in the HeLa cell line and the highest in the SiHa cell line,both of which were statistically significant compared to other cell lines(P<0.001).In SiHa cell line,compared with NC group,shRNA1,shRNA2 and shRNA3 lentiviruses significantly reduced the expression level of circNRIP1,among which shRNA2 had the highest knockdown ability.In HeLa cell line,compared with OV-NC group,SHRNA2 had the highest knockdown ability.The expression level of circNRIP1 in OV-cNRIP1 group was significantly higher(P<0.01).The MTS assay results showed that in HeLa cell lines,the proliferation ability of HeLa cells in the OV-cNRIP1 group was significantly higher than that in OV-NC group(P<0.001).In SiHa cell lines,the proliferation capacity of SiHa cells in sh-cNRIP1 group was lower than that in sh-NC group(P<0.001).The results of scratch assay showed that the SiHa cell migration ability of sh-cNRIP1 group was lower than that of sh-NC group(P<0.001).In HeLa cell line,the migration ability of HeLa cells in OV-cNRIP1 group was significantly enhanced compared with OV-NC group(P<0.001).The Transwell Matrigel assay esults showed that in the SiHa cell line,compared with the sh-NC group,the number of SiHa cells membrane penetration in the sh-cNRIP1 group was significantly reduced(P<0.001).In the HeLa cell line,compared with the OV-NC group,the number of

关 键 词：宫颈癌 circNRIP1 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R737.33[医药卫生—肿瘤]