RECQL4在胃癌中的表达及其与预后、免疫细胞浸润水平、药物敏感性之间的关系  

The expression of RECQL4 in gastric cancer and its relationship with prognosis,immune cell infiltration levels and drug sensitivity

作　　者：姜德培 钟剑锋 张伟康 胡志耀 文学 王新友[2] 陈泓磊 Jiang Depei;Zhong Jianfeng;Zhang Weikang;Hu Zhiyao;Wen Xue;Wang Xinyou;Chen Honglei(Gastrointestinal Endoscopy Center,the Eighth Affiliated Hospital of Sun Yat-sen University,Shenzhen 518033,Guangdong,China;Gastrointestinal Surgery,the Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,Guangdong,China)

机构地区：[1]中山大学附属第八医院(深圳福田)消化内镜中心,广东深圳518033 [2]中山大学附属第六医院胃外科,广东广州510655

出　　处：《消化肿瘤杂志（电子版）》2024年第1期50-57,共8页Journal of Digestive Oncology(Electronic Version)

基　　金：深圳市福田区卫生健康系统科研项目(FIWS2021056,FIWS2023001);广东省基础与应用基础研究基金项目(2021A1515011630)。

摘　　要：目的探究RECQL4在胃癌组织中的表达情况,并研究其与预后、免疫细胞浸润水平以及药物敏感性之间的关系。方法通过TIMER 2.0数据库分析RECQL4在不同肿瘤中的表达差异性。通过GEPIA2数据库、GEO数据库分析RECQL4在胃癌组织中的表达水平。通过Kaplan-Meier plotter数据库对RECQL4在胃癌患者预后中的价值进行生存分析评估。通过TIMER 2.0数据库评估RECQL4的表达与免疫细胞浸润水平的相关性。应用RNAactDrug数据库揭示RECQL4分子与药物敏感性之间的关系。结果RECQL4在胃癌、结直肠癌、食管癌等肿瘤组织中高表达;RECQL4的高表达与胃癌的较差预后相关。胃癌中RECQL4 mRNA表达水平与B细胞、CD8+T细胞、CD4^(+)T细胞、树突状细胞、中性粒细胞及巨噬细胞浸润水平呈负相关。治疗胃癌的常用药物伊立替康、5-氟尿嘧啶、紫杉醇及多西他赛的敏感性均与RECQL4相关。结论RECQL4在胃癌组织中高表达,且可能与多种化疗药物敏感性相关,可作为预后不良的标志物。RECQL4高表达可抑制免疫细胞浸润,可能参与了肿瘤的免疫逃逸,促进肿瘤发展。Objective To investigate the expression of RECQL4 in gastric cancer tissues and its association with prognosis,immune cell infiltration levels and drug sensitivity.Method The differential expression analysis of RECQL4 across various tumor types was conducted using TIMER 2.0 database.The GEPIA2 and GEO database were utilized to analyze the expression level of the RECQL4 in gastric cancer tissues.Survival analysis utilizing Kaplan-Meier plotter database was performed to evaluate the prognostic significance of RECQL4 in gastric cancer patients.Furthermore,the association between RECQL4 expression and immune cell infiltration levels was evaluated by TIMER 2.0 database.Additionally,RNAactDrug database was employed to explore potential associations between RECQL4 molecules and drug sensitivity.Result Compared with normal tissues,the expression of RECQL4 is significantly upregulated in gastric cancer,colorectal cancer,and esophageal cancer.The overexpression of RECQL4 is associated with a poor prognosis in gastric cancer patients.Moreover,the mRNA expression level of RECQL4 in gastric cancer demonstrates a negative correlation with the infiltration levels of B cells,CD8+T cells,CD4^(+)T cells,dendritic cells,neutrophils,and macrophages.Additionally,the sensitivity to irinotecan,5-fluorouracil,paclitaxel,and docetaxel utilized for treating gastric cancer is all closely linked to RECQL4.Conclusion RECQL4 is highly expressed in gastric cancer tissues and may be associated with a variety of chemotherapy sensitivities,which can be used as a marker of poor prognosis.High expression of RECQL4 can inhibit immune cell infiltration,which may be involved in tumor immune escape and promote tumor development.

关 键 词：胃癌 解旋酶 临床预后 免疫浸润 药物敏感性 

分 类 号：R735.2[医药卫生—肿瘤]