冻融季温带森林土壤真菌群落结构及功能类群对不同土壤有机碳输入的响应  

Soil fungal community structure and functional group in response to different soil organic carbon inputs in the temperate forest during the freeze-thaw season

作　　者：陈羽彤 张利敏[1] 王思琪 周利军 CHEN Yutong;ZHANG Limin;WANG Siqi;ZHOU Lijun(Heilongjiang Province Key Laboratory of Geographical Environment Monitoring and Spatial Information Service in Cold Regions,School of Geographical Science,Harbin Normal University,Harbin 150025,China;College of Agriculture and Hydraulic Engineering,SuiHua University,Suihua 152061,China)

机构地区：[1]哈尔滨师范大学寒区地理环境监测与空间信息服务黑龙江省重点实验室,哈尔滨师范大学地理科学学院,哈尔滨150025 [2]绥化学院,农业与水利工程学院,绥化152061

出　　处：《生态学报》2024年第6期2244-2255,共12页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31670619,42371119);黑龙江省留学归国人员科学基金(LC2018011);黑龙江省自然科学基金项目(LH2019D018)。

摘　　要：根系与凋落物有机碳输入变化对土壤生物群落的影响研究是目前学术界关注的热点问题,但冻融季不同有机碳输入方式将对土壤真菌群落结构及功能类群产生何种影响尚不明确。土壤真菌群落是调节森林生态系统稳定性的重要因素,有助于维持生态系统生产力时间尺度的稳定性。为了探索冻融季温带森林土壤真菌群落对控制根系和凋落物有机碳输入方式的响应特征,通过在帽儿山生态站设置4种碳源输入控制处理植物残体添加去除(DIRT):去除凋落物仅根系输入处理、去除根系仅凋落物输入处理、无碳源输入处理和同时进行根系与凋落物输入处理,采用ITS rDNA高通量测序技术和FUNGuild功能预测平台,来分析控制根系和凋落物有机碳输入方式对温带森林土壤真菌群落结构和功能类群的影响。研究结果显示:(1)不同有机碳输入方式改变了土壤真菌类群的多度:与自然生长状态下有机碳输入方式相比,根系有机碳输入比凋落物有机碳输入对土壤真菌类群多度影响更明显,去除根系碳源输入处理使真菌群落中子囊菌门含量升高19.52%,担子菌门含量下降16.77%。(2)有机碳输入方式对土壤真菌群落功能类群产生影响:与自然生长状态下有机碳输入方式相比,去除根系碳源输入处理使群落内腐生营养型真菌、病原营养型真菌数量占比增加9.79%、1.22%;去除凋落物碳源输入处理下群落内腐生营养型、病原营养型真菌数量占比下降7.58%、0.85%。(3)有机碳输入方式对真菌群落结构的影响与土壤理化环境因子有关,微生物氮的含量是主导土壤真菌群落结构出现显著差异的关键因子。以上结果表明有机碳输入方式对土壤真菌群落结构及功能类群有重要调控作用,研究结果丰富了温带森林土壤微生物群落的研究内容,为研究土壤微生态环境的变化对生态系统过程的影响提供理论支持和参�Research on the influence of change of organic carbon input from aboveground litter and belowground roots on soil biological community is a topical issue in academic circles at present.However,it is unclear about the influence of organic carbon input methods on soil fungal community structure and functional group during the freeze-thaw season.The soil fungal community is an important factor in regulating the stability of forest ecosystem,which help to maintain the stability of ecosystem productivity timescales.In order to investigate the response characteristics of the soil fungal community to organic carbon input from aboveground litter and belowground root in freeze-thaw seasonal temperate forest,this study used ITS rDNA high-throughput sequencing technology and the FUNGuild functional prediction platform.Four different carbon input treatments were set up in Maoershan Ecological Station(Detritus input and removal treatments,DIRT),including litter removal(NL),root removal(NR),litter and root removal(NLR)and control(CK).We analyzed the influence of controlling roots and litter on fungal community structure and functional groups in temperate forest soil.The result showed that:(1)different organic carbon input patterns altered the relative abundance of the soil fungal community.Root organic carbon input had more obvious effects on the relative abundance of soil fungal communities than litter inputs.Relative abundance of the Ascomycota was 19.52% higher and relative abundance of the Basidiomycota was 16.77% lower than those in the control treatment,respectively.(2)The mode of organic carbon input had a significant effect on the functional group of the soil fungal community.The proportion of Saprotroph fungi and Pathotroph fungi were 9.79% and 1.22% higher in root removal treatment,at the same time which were 7.58% and 0.85% lower than those in the control treatment.(3)The influence of organic carbon input mode on fungal community structure was related to soil physical and chemical environmental factors.The content o

关 键 词：植物残体添加去除 FUNGuild功能预测 高通量测序 alpha多样性 冻融季 

分 类 号：S714.3[农业科学—林学]