制何首乌产地加工炮制一体化新方法的优势分析  

Analysis on Advantages of New Integration Processing Method in Producing Area of Polygoni Multiflori Radix Praeparata

作　　者：辛雪颖 景佳麟[1,2] 高双荣 张江山[3] 龚千锋 骆璐[1] 李娆娆 刘婷[1] XIN Xueying;JING Jialin;GAO Shuangrong;ZHANG Jiangshan;GONG Qianfeng;LUO Lu;LI Raorao;LIU Ting(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Jingde Traditional Chinese Medicine Co.Ltd.,Jingdezhen 333302,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]江西中医药大学药学院,南昌330004 [3]江西景德中药股份有限公司,江西景德镇333302

出　　处：《中国实验方剂学杂志》2024年第8期167-175,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院科技创新工程项目(CI2021A03707);北京市自然科学基金项目(7112097)。

摘　　要：目的:分析产地加工炮制一体化新方法与传统方法对制何首乌成分和药理作用的影响,阐释新方法所制备饮片的“减毒增效”优势。方法:取道地产区鲜何首乌,分别采用产地加工炮制一体化新方法(压力0.1 MPa,120℃隔水加黑豆汁蒸制10.5 h)和传统方法(隔水加黑豆汁蒸制36 h)进行炮制。2种方法均在炮制过程取样,其中新方法取样时间点依次为0.5、3、5.5、8、10.5 h,传统方法每隔4 h取样1次(共炮制36 h)。采用高效液相色谱法(HPLC)建立指纹图谱并确定共有峰,蒽酮-硫酸比色法测定多糖类成分含量,按2020年版《中华人民共和国药典》何首乌与制何首乌“含量测定”项下方法检测不同炮制品中蒽醌类及二苯乙烯苷含量。在药效实验中,90只SD大鼠随机分为空白组,生品、常压24 h炮制品、常压30 h炮制品、加压8 h炮制品的低、高剂量组(4.1、16.5 g·kg^(-1)),每组10只,雌雄各半。均连续灌胃给药29 d,1次/d。末次给药后腹主动脉取血,分离血清,称取大鼠体质量、肝脏质量,计算脏器指数;采用苏木素-伊红(HE)染色观察大鼠肝脏组织病理变化,生化法检测血清中肝功能指标天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰基转移酶(GGT)、乳酸脱氢酶(LDH)的水平及脑组织中氧化指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的水平。结果:在生何首乌、新方法和传统方法制何首乌指纹图谱中共确定了14个共有峰,并指认其中3个色谱峰分别为二苯乙烯苷、大黄素、大黄素甲醚。各炮制品在0~25 min特征峰面积变化较为明显,表明不同炮制方法对制何首乌中极性较高的成分含量存在影响,2种方法的变化趋势相似,以新方法变化程度较高。含量测定结果显示,与传统方法比较,新方法所得炮制品中补益类成分多糖含量升高、肝损伤类成分二苯乙烯苷�Objective:To analyze the effects of new integration processing method in producing area and traditional method on the composition and pharmacological action of Polygoni Multiflori Radix Praeparata(PMRP),and to illustrate the advantages of toxicity reducing and efficacy enhancing of the decoction pieces prepared by the new method.Method:Fresh Polygoni Multiflori Radix(PMR)was taken from Dao-di producing area,and was processed by new integration processing method in producing area(steaming with black bean juice under pressure of 0.1 MPa and temperature at 120℃for 10.5 h)and traditional method(steaming with black bean juice under water for 36 h),respectively.Samples were collected during the processing process of the two methods,For new method,the samples were collected at 0.5,3,5.5,8,10.5 h,separately.For traditional method,the samples were collected every 4 h.High performance liquid chromatography(HPLC)was used to establish fingerprint and identify common peaks,the content of polysaccharides was determined by anthrone-sulfuric acid colorimetry at 627 nm,and the contents of anthraquinones and stilbene glycosides in different processed products were determined according to the methods under the item of determination of PMR and PMRP in the 2020 edition of Chinese Pharmacopoeia.In pharmacological experiments,90 SD rats were randomly divided into 9 groups with 10 in each group(half of male and half of female),including the blank group,and raw products,24 h processed products under atmospheric pressure,30 h processed products under atmospheric pressure,8 h processed products under high pressure groups with low and high dosages(4.125,16.5 g·kg^(-1)).Rats were given the drug by gavage for 29 d with once a day,blood was collected from the abdominal aorta after the last administration,and the serum was isolated,the body mass and liver mass of rats were weighed and the organ index was calculated.The pathological change of liver tissue was observed by hematoxylin-eosin(HE)staining,and biochemical methods were used to detec

关 键 词：何首乌 产地加工炮制一体化 主成分含量 指纹图谱 减毒增效 蒽醌 二苯乙烯苷 抗氧化 肝损伤 

分 类 号：R22[医药卫生—中医基础理论] R943.1[医药卫生—中医学] R28[理学—分析化学] O657[理学—化学]