异源三聚体胶原多肽:设计、表征和应用  

Heterotrimeric collagen peptides:design,characterization,and applications

作　　者：权思齐 张蓝月 张子勇 孙秀霞 肖建喜[1] Siqi Quan;Lanyue Zhang;Ziyong Zhang;Xiuxia Sun;Jianxi Xiao(College of Chemistry and Chemical Engineering,Lanzhou University,Lanzhou 730000,China;School of Pharmacy,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学化学化工学院,兰州730000 [2]兰州大学药学院,兰州730000

出　　处：《中国科学：化学》2024年第3期370-384,共15页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:22074057);国家青年科学基金(编号:20230790);甘肃省重点研发计划(编号:20YF3FA025)资助项目。

摘　　要：胶原蛋白是人体内含量最丰富的蛋白质,其中异源三聚体胶原蛋白含量超过90%.异源三聚体胶原蛋白任意一条肽链的甘氨酸发生突变都会引发遗传疾病.胶原蛋白分子量大且结构复杂,在分子层面研究胶原蛋白存在挑战.胶原多肽的分子量小且结构易于控制,被广泛应用于仿生胶原蛋白.此外异源三聚体胶原多肽可以精确模拟Ⅰ型和Ⅳ型等异源三聚体胶原蛋白的组成、结构和功能.本综述介绍了异源三聚体胶原多肽的设计方法、表征手段以及应用.首先总结了共价键连接和非共价相互作用这两种构建异源三聚体胶原多肽的策略;其次概述了异源三聚体胶原多肽的肽链组成、三螺旋结构以及热稳定性的表征方法;接着介绍了异源三聚体胶原多肽在模拟胶原蛋白肽链排列、仿生胶原蛋白纤维结构以及研究成骨不全症机制等方面的应用;最后展望了功能化的异源三聚体胶原多肽的开发及其在生物医学领域内的应用前景.Collagen is the most abundant protein in the human body.Over 90%of its total content is heterotrimers.Mutation of glycine in any polypeptide chain of natural heterotrimeric collagen will lead to genetic diseases.Due to the large molecular weight and complex structure of collagen,it is challenging to investigate collagen at the molecular level.Collagen peptides have been widely contributed to mimic collagen.Due to their small molecular weight and easily controlled structure,heterotrimeric collagen peptides can precisely mimic the chain composition,structure,and function of heterotrimeric collagen,such as type I and type IV collagen.This review describes in detail the design,characterization,and applications of heterotrimeric collagen peptides.Firstly,two strategies for constructing heterotrimeric collagen peptides have been summarized,involving covalent bonding and non-covalent interactions.Secondly,the methods for characterizing chain composition,triple helical structure,and thermal stability of heterotrimeric collagen peptides have been outlined.Furthermore,the application areas of heterotrimeric collagen peptides in simulating the chain registers and fibrous structure of collagen,as well as investigating the mechanisms of osteogenesis imperfecta,have been introduced.Finally,an outlook on the development of functionalized heterotrimeric collagen peptides and their potential applications in the field of biomedical research has been proposed.

关 键 词：胶原蛋白 多肽 异源三聚体 设计 表征 应用 

分 类 号：O629.72[理学—有机化学]