茄子全基因组InDel变异特征及分子标记开发和应用  

Variation Characteristics of Insertion-Deletion(InDel)and Molecular Markers Development and Application in Eggplant Based on Whole Genome Re-sequencing Data

作　　者：尤园园 王帅[2] 方莹莹 齐诚 李淑培 张映[1] 陈钰辉[1] 刘伟[3] 刘富中[1] 舒金帅[1] YOU Yuanyuan;WANG Shuai;FANG Yingying;QI Cheng;LI Shupei;ZHANG Ying;CHEN Yuhui;LIU Wei;LIU Fuzhong;SHU Jinshuai(State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Horticultural Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004,China;Shouguang Vegetable Research and Development Center,Chinese Academy of Agricultural Sciences,Shouguang,Shandong 262704,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [2]河北科技师范学院园艺科技学院,河北秦皇岛066004 [3]中国农业科学院寿光蔬菜研发中心,山东寿光262704

出　　处：《园艺学报》2024年第3期520-532,共13页Acta Horticulturae Sinica

基　　金：中央级公益性科研院所基本科研业务费专项(IVF-BRF2022003,IVF-BRF2021006);中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS);重庆市人民政府与中国农业科学院战略合作资助项目;中蔬生物科技(寿光)有限公司企业研发项目(ZSSW-2023007,ZSSW-2023008);农业农村部园艺作物生物学与种质创制重点实验室项目。

摘　　要：选取4份圆茄(Solanum melongena L.)高代自交系进行全基因组重测序,鉴定出145 407个InDel,12号染色体InDel数最少(7 752),1号最多(19 738)。筛选出7 959个长度大于10bp的InDel位点,挑选均匀分布在基因组上的144个设计InDel引物并进行验证。利用26份茄子材料对144对InDel引物进行筛选,63对表现出稳定的多态性,比例为43.75%;主要等位基因频率为0.4038~0.9808,平均为0.7659;基因多样性指数(Hs)为0.0377~0.6501,平均为0.3217;多态性信息含量(PIC)为0.0370~0.5750,平均为0.2663,42个标记多态性信息含量大于0.200,多态性较好。当遗传距离为0.235时,将26份不同类型的茄子材料划分为2个类群。利用多态性信息含量高的前10个InDel标记构建了26份茄子材料DNA指纹图谱。另外,选择在亲本间具有多态性的InDel标记用于4个杂交组合的F1代真实性鉴定,鉴定结果表明3个为真实杂交种,与田间鉴定结果吻合。Four high generation inbred lines of eggplant(Solanum melongena L.)were selected for whole genome re-sequencing,and 145407 InDels were identified.The number of InDel on chromosome 12 was the least(7752)and on chromosome 1 was the most(19738).There were 7959 InDel with a length greater than 10 bp,and 144 InDels loci uniformly distributed on the genome were selected to be designed as InDel primers and verified.A total of 144 InDel primers were screened using 26 eggplant samples,63 primers showed stable polymorphism,and the polymorphism ratio was 43.75%.The main allele frequencies ranged from 0.4038 to 0.9808,with an average of 0.7659.Genetic diversity index(H s)ranged from 0.0377 to 0.6501,with an average of 0.3217.The polymorphism information content(PIC)ranged from 0.0370 to 0.5750,with an average of 0.2663,and the PIC of 43 markers was greater than 0.200,indicating good polymorphism.When the genetic distance was 0.235,26 different types of eggplant materials were divided into two groups.The DNA fingerprints of 26 eggplant materials were constructed using the first ten InDel markers with high polymorphism information content.In addition,InDel markers with polymorphism among parents were selected for F 1 generation authenticity identification of four hybrid combinations,and three real hybrids were identified.The results from purity test of four hybrid combinations from InDel markers matched well with those from field experiments.

关 键 词：茄子 INDEL标记 遗传多样性 指纹图谱 F1代真实性鉴定 

分 类 号：S641.1[农业科学—蔬菜学]