副干酪乳酪杆菌Glory LP16对调节小鼠肠道菌群功能的影响  

Effect of Lacticaseibacillus paracasei Glory LP16 in Regulating Intestinal Flora in Mice

作　　者：杨柳 项芳琴 王海霞[1] 姜海莘 李柏良[1] YANG Liu;XIANG Fangqin;WANG Haixia;JIANG Haixin;LI Bailiang(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《食品研究与开发》2024年第7期10-16,共7页Food Research and Development

基　　金：国家自然科学基金面上项目(32072190);中国博士后科学基金面上项目(2022M721071)。

摘　　要：为探究副干酪乳酪杆菌Glory LP16调节肠道菌群的功能,该实验3组小鼠分别灌胃1.6×10^(6)、1.6×10^(7)、1.6×10^(8) CFU副干酪乳酪杆菌Glory LP16,测定干预14 d后小鼠体质量、肠道菌群、结肠组织病理学变化、肠道屏障、肠道通透性及短链脂肪酸含量。结果显示,干预14 d后,各组小鼠粪便中双歧杆菌、乳杆菌含量均显著增加(P<0.05),产气荚膜梭菌含量显著下降(P<0.05),而肠杆菌和肠球菌的含量无显著变化(P>0.05);高剂量组副干酪乳酪杆菌Glory LP16显著降低血清脂多糖(lipopolysaccharide,LPS)含量和D⁃乳酸含量(P<0.05),显著上调黏蛋白1、黏蛋白2和紧密连接蛋白(Claudin⁃1、Occludin、ZO⁃1)基因的表达水平,粪便中乙酸、丙酸和丁酸含量显著增加,且小鼠肠道无明显水肿和炎症细胞浸润,对小鼠生长无不良影响。This paper investigated the function of Lacticaseibacillus paracasei Glory LP16 in regulating intestinal microbiota.Three groups of mice were orally administered with L.paracasei Glory LP16 at doses of 1.6×10^(6),1.6×10^(7 )CFU,and 1.6×10^(8) CFU,respectively.After 14 d of intervention,body weight,intestinal microbiota,co⁃lonic histopathological changes,intestinal barrier,intestinal permeability,and short⁃chain fatty acid of the mice were measured.The results showed that after the 14 d intervention,Bifidobacteria and Lactobacilli in the feces of all groups of mice increased(P<0.05),while Clostridium perfringens decreased(P<0.05),and there were no significant changes in Enterobacter and Enterococcus species(P>0.05).High⁃dose L.paracasei Glory LP16 down⁃regulated serum lipopolysaccharide(LPS)and D⁃lactic acid(P<0.05),up⁃regulated the expres⁃sions of mucin 1(MUC1),mucin 2(MUC2),and tight junction proteins(Claudin⁃1,Occludin,ZO⁃1)genes,and significantly increased acetate,propionate,and butyrate in the feces.The intestinal tract of mice had no obvious edema and inflammatory cell infiltration,and no adverse effect was found on their growth.

关 键 词：副干酪乳酪杆菌Glory LP16 肠道菌群 组织病理 肠道屏障 短链脂肪酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]