miR-145-5P通过调节RhoA蛋白表达抑制U251胶质瘤细胞的功能  

miR-145-5P Inhibits the Function of U251 Glioma Cells by Regulating RhoA Protein

作　　者：王军成[1] 赵岳阳 刘海波[3] 张斌[3] WANG Juncheng;ZHAO Yueyang;LIU Haibo;ZHANG Bin(Department of Neurosurgery,People’s Hospital of Ningxia Hui Autonomous Region,Third Clinical Medical College of Ningxia Medical University,Yinchuan 750001,China;Department of Oncology,People’s Hospital of Ningxia Hui Autonomous Region,Third Clinical Medical College of Ningxia Medical University,Yinchuan 750001,China;Department of Neurosurgery,the General Hospital of Ningxia Medical University,First Clinical Medical College of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏回族自治区人民医院神经外科,宁夏医科大学第三临床医学院,银川750001 [2]宁夏回族自治区人民医院肿瘤科,宁夏医科大学第三临床医学院,银川750001 [3]宁夏医科大学总医院神经外科,宁夏医科大学第一临床医学院,银川750004

出　　处：《宁夏医科大学学报》2024年第2期125-129,181,共6页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金项目(2021AAC03313,2021AAC03398);宁夏医科大学科学研究项目(XM2020087);宁夏回族自治区人民医院国家自然科学基金预实验项目(2021GZRYSY017);2021年第六批宁夏回族自治区青年科技人才托举工程;宁夏回族自治区卫健委重点项目(2019-NW-017)。

摘　　要：目的探究miR-145-5P对U251胶质瘤细胞增殖、侵袭影响及作用机制。方法使用miR-145-5P mimic体外干预U251胶质瘤细胞,分为miR-145-5p过表达[miR-145-5p(+)]组、对照(NC)组。使用CCK-8实验、划痕愈合实验、Transwell实验检测miR-145-5P对U251细胞增殖、迁移、侵袭的影响,免疫荧光实验检测miR-145-5P对U251胶质瘤细胞骨架形成的影响,Western blot实验检测各组细胞中RhoA蛋白表达量的变化。结果与NC组相比,CCK-8实验、划痕愈合实验、Transwell实验显示,miR-145-5p(+)组U251细胞的OD值、划痕愈合率、迁移/侵袭细胞数均明显减少(P均<0.05);免疫荧光实验显示,miR-145-5p过表达后细胞形态由梭形变为圆形,丝状伪足及片状伪足皱缩;Western blot实验结果显示,miR-145-5p过表达后U251细胞中RhoA蛋白表达量减少(P<0.05)。结论miR-145-5P通过调节RhoA蛋白表达量,进而影响胶质瘤细胞增殖、迁移、侵袭等生物学功能。Objective To investigate the effect and mechanism of miR-145-5P on the proliferation and invasion of U251 glioma cells.Methods U251 glioma cells were intervened in vitro with miR-145-5P mimic,which were divided into the miR-145-5p overexpression[miR-145-5p(+)]group and the control(NC)group.The effects of miR-145-5p on proliferation,migration and invasion of U251 cells were detected by CCK-8,scratch healing experiment and Transwell experiment.The effects of miR-145-5p on cytoskeleton formation of U251 cells were detected by immunofluorescence experiment,and the expression of RhoA protein in each group were detected by Western blot.Results Compared with the NC group,The results of CCK-8 experiment,scratch healing experiment,and Transwell experiment showed that the OD value,scratch healing rate,and the number of migrating/invading cells of U251 in the miR-145-5p(+)group were significantly reduced(P all<0.05).The immunofluorescence experiments showed that the cell morphology changed from spindle shaped to circular,with filamentous pseudopodia and patchy pseudopodia wrinkling after the overexpression of miR-145-5p.Western blot experiments showed that the expression of RhoA protein reduced in U251 cells after the overexpression of miR-145-5p(P<0.05).Conclusion miR-145-5p regulates the expression of RhoA protein,thereby affecting the biological functions of glioma cells such as proliferation,migration,and invasion.

关 键 词：RHOA蛋白 胶质瘤 增殖 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]