基于TCGA数据库分析SSBP1基因在肝细胞癌中的表达及预后  

Expression and Prognosis of Mitochondrial Single-Stranded DNA-Binding Protein 1 Gene in Hepatocellular Carcinoma Based on TCGA Dataset

作　　者：林亚茹 杨玺 李锦松 刘如爱 普元倩 李建康 余敏 熊伟 LIN Yaru;YANG Xi;LI Jinsong;LIU Ru’ai;PU Yuanqian;LI Jiankang;YU Min;XIONG Wei(Department of Biochemistry and Molecular Biology,School of Basic Medicine,Dali University,Dali 671000,China;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical,College of Pharmacy,Dali 671000,China;Yunnan University Key Laboratory of Clinical Biochemistry,Dali671000,China;Laboratory of Biochemistry and Molecular Biology,School of Life Sciences,Yunnan University,Dali Yunnan 650091,China)

机构地区：[1]大理大学基础医学院生物化学与分子生物学教研室,大理671000 [2]云南省昆虫生物医药研发重点实验室,大理671000 [3]云南省高校临床生物化学检验重点实验室,大理671000 [4]云南大学生命科学学院生物化学与分子生物学实验室,昆明650091

出　　处：《宁夏医科大学学报》2024年第2期146-152,共7页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(82160516);云南省应用基础研究专项面上项目(202201AT070004,202301AT070023);云南省地方本科高校基础研究联合专项面上项目(202101BA070001-226);云南省高校地方本科高校基础研究联合专项青年项目(2022);云南省昆虫生物医药研发重点实验室开放课题(AG2022003,AP2022006);云南省教育厅科学研究基金研究生项目(2023Y0949)。

摘　　要：目的利用癌症基因组图谱(TCGA)数据库研究SSBP1基因在肝细胞癌(HCC)组织中的表达情况,并探讨SSBP1基因表达量与患者临床病理特征及预后的相关性。方法基于TCGA数据库提取374例HCC组织及50例正常肝组织中SSBP1基因表达量数据、患者的临床病理参数资料,利用R 4.1.2分析SSBP1在HCC中的表达情况及其与HCC患者的临床病理特征和预后的相关性,通过基于基因表达水平值的交互式分析平台(GEPIA)数据库和R 4.1.2联合分析SSBP1在HCC各临床病理分期间的表达差异,利用TIMER数据库分析SSBP1表达与免疫细胞浸润的相关性,通过R软件pROC程序包制作ROC生存曲线评估SSBP1表达量对HCC的诊断效果。结果TCGA数据库分析表明,无论是配对样本还是非配对样本,SSBP1 mRNA在HCC组织中的表达水平均高于正常肝组织(P均<0.001),且表达水平与性别和M分期有关(P均<0.05),与年龄、T分期、AJCC分期等无关(P均>0.05)。进一步进行单因素和多因素Cox回归分析发现,SSBP1高表达是影响HCC患者总生存期(OS)的独立危险因素(HR=1.713,P=0.016)。免疫细胞浸润分析发现,HCC组织中SSBP1表达与幼稚B细胞浸润水平呈负相关,而与记忆B细胞和巨噬细胞呈正相关(|r|>0.2,P均<0.05)。ROC曲线分析显示,SSBP1 mRNA表达水平对HCC的早期诊断和预后均具有良好的评估价值(AUC>0.50)。结论SSBP1 mRNA在HCC中的表达增高,且高表达是预后不良的独立危险因素,同时SSBP1对HCC具有良好的诊断价值,可成为HCC患者预后判断一种潜在的分子标志物以及免疫治疗的潜在靶点。Objective To study the expression of SSBP1 gene in hepatocellular carcinoma(HC)tissues using the cancer genome atlas(TCGA)database and to investigate the correlation between SSBP1 gene expression and clinicopathologic features and prognosis of patients.Methods Based on TCGA database,we extracted the relevant data,clinicopathological parameters of SSBP1 gene mRNA expression in 374 cases of HCC tissues and 50 cases of normal liver tissues,analyzed the expression of SSBP1 in HCC.By using the GEPIA database and R 4.1.2,the expression differences of SSBP1 among different clinical and pathological stages of HCC were analyzed.The TIMER database was used to analyze the correlation between SSBP1 expression and immune cell infiltration.Finally,the ROC survival curve was created using the R software pROC package to evaluate the diagnostic effect of SSBP1 expression on HCC.Results TCGA database analysis showed that the expression level of SSBP1 mRNA in HCC tissues was higher than that in normal tissues(P all<0.001)in both matched and unmatched samples,and the expression level was correlated with gender and M stage(P all<0.05),but not with age,T stage,AJCC stage(P all>0.05).Further Cox regression analysis revealed that high expression of SSBP1 was an independent risk factor for OS in HCC patients(HR=1.713,P=0.016).Immunocell infiltration analysis showed that SSBP1 expression in HCC was negatively correlated with the invasion level of naive B cells,but positively correlated with memory B cells and macrophages(|r|>0.2,P all<0.05).ROC curve analysis uncovered that expression level of SSBP1 mRNA exhibited well diagnostic value for in HCC early diagnosis and prognosis(AUC>0.50).Conclusion SSBP1 mRNA expression is increased in HCC,and high expression is an independent risk factor for poor prognosis.Meanwhile,SSBP1 has a good diagnostic value for HCC,and it can be a potential molecular marker for prognosis judgment of HCC patients as well as a potential target for immunotherapy.

关 键 词：线粒体单链DNA结合蛋白 癌症基因组图谱 肝细胞癌 生物标志物 预后 

分 类 号：R735.7[医药卫生—肿瘤]