Hepatic and renal effects of oral stingless bee honey in a streptozotocin-induced diabetic rat model  

作　　者：Suriati Mohd Nasir Anis Farihan Ismail Tuan Salwani Tuan Ismail Wan Faiziah Wan Abdul Rahman Wan Amir Nizam Wan Ahmad Tengku Ahmad Damitri Al-Astani Tengku Din Kuttulebbai Nainamohammed Salam Sirajudeen 

机构地区：[1]Department of Chemical Pathology,School of Medical Sciences,Universiti Sains Malaysia,Kota Bharu 16150,Kelantan,Malaysia [2]Endocrinology Laboratory,Department of Chemical Pathology,School of Medical Sciences,Universiti Sains Malaysia,Kota Bharu 16150,Kelantan,Malaysia [3]Department of Pathology,School of Medical Sciences,Universiti Sains Malaysia,Kota Bharu 16150,Kelantan,Malaysia [4]Biomedicine Program,School of Health Sciences,Health Campus,Universiti Sains Malaysia,Kota Bharu 16150,Kelantan,Malaysia [5]Department of Basic Medical Sciences,Kuliyyah of Medicine,International Islamic University Malaysia,Bandar Indera Mahkota,Kuantan 25200,Pahang,Malaysia

出　　处：《World Journal of Experimental Medicine》2024年第1期113-123,共11页世界实验医学杂志

基　　金：Ministry of Higher Education,Malaysia for Fundamental Research Grant Scheme FRGS/1/2019/SKK06/USM/03/6,No.291983-329281.

摘　　要：BACKGROUND Diabetes is known damage the liver and kidney,leading to hepatic dysfunction and kidney failure.Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications.However,the effect of stingless bee honey(SBH)administration in relieving liver and kidney damage in diabetes has not been well-studied.AIM To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced(STZ;55 mg/kg)diabetic Sprague Dawley rats.METHODS The rats were grouped as follows(n=6 per group):non-diabetic(ND),untreated diabetic(UNT),metformin-treated(MET),and SBH+metformin-treated(SBME)groups.After successful diabetic induction,ND and UNT rats were given normal saline,whereas the treatment groups received SBH(2.0 g/kg and/or metformin(250 mg/kg)for 12 d.Serum biochemical parameters and histological changes using hematoxylin and eosin(H&E)and periodic acid–Schiff(PAS)staining were evaluated.RESULTS On H&E and PAS staining,the ND group showed normal architecture and cellularity of Bowman’s capsule and tubules,whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane.The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining,but the two were similar on PAS staining.Likewise,the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining,but it was comparable to the SBH and ND groups on PAS staining.UNT diabetic rats had tubular hydropic tubules,which were smaller than other groups.Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group.Conversely,the UNT group had high glucose levels,which subsequently increased MDA levels,ultimately leading to liver damage.SBH treatment reduced this damage,as evidenced by having the lowest fasting glucose,serum alanine transaminase,aspartate transaminase,and alkaline phosphatase levels compared to other groups,although the

关 键 词：Diabetes STREPTOZOTOCIN Stingless bee honey Hematoxylin and eosin Periodic acid-Schiff Liver Kidney 

分 类 号：R58[医药卫生—内分泌]