Curcumin inhibits the growth and invasion of gastric cancer by regulating long noncoding RNA AC022424.2  

作　　者：Bin-Sheng Wang Chen-Li Zhang Xiang Cui Qiang Li Lei Yang Zhi-Yun He Ze Yang Miao-Miao Zeng Nong Cao 

机构地区：[1]Department of General Surgery,First Hospital of Lanzhou University,Lanzhou 730000,Gansu Province,China [2]School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,Gansu Province,China [3]Third Department of General Surgery,First Hospital of Lanzhou University,Lanzhou 730000,Gansu Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第4期1437-1452,共16页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Supported by the Natural Science Foundation of Gansu Province,China,No.20JR5RA356 and No.22JR5RA511;the Lanzhou City Chengguan District Science and Technology Planning Project,No.2016-7-17.

摘　　要：BACKGROUND Gastric cancer,characterized by a multifactorial etiology and high heterogeneity,continues to confound researchers in terms of its pathogenesis.Curcumin,a natural anticancer agent,exhibits therapeutic promise in gastric cancer.Its effects include promoting cell apoptosis,curtailing tumor angiogenesis,and enhancing sensitivity to radiation and chemotherapy.Long noncoding RNAs(lncRNAs)have garnered significant attention as biomarkers for early screening,diagnosis,treatment,and drug response because of their remarkable specificity and sensitivity.Recent investigations have revealed an association between aberrant lncRNA expression and early diagnosis,clinical staging,metastasis,drug sensitivity,and prognosis in gastric cancer.A profound understanding of the intricate mechanisms through which lncRNAs influence gastric cancer develop-ment can provide novel insights for precision treatment and tailored management of patients with gastric cancer.This study aimed to unravel the potential of curcumin in suppressing the malignant behavior of gastric cancer cells by upregu-lating specific lncRNAs and modulating gastric cancer onset and progression.AIM To identify lncRNAs associated with curcumin treatment and investigate the role of lncRNA AC022424.2 in the effects of curcumin on gastric cancer cell apoptosis,proliferation,and invasion.Furthermore,these findings were validated in clinical samples.METHODS The study employed CCK-8 assays to assess the impact of curcumin on gastric cancer cell proliferation,flow cytometry to investigate its effects on apoptosis,and scratch and Transwell assays to evaluate its influence on the migration and invasion of BGC-823 and MGC-803 cells.Western blotting was used to gauge changes in the protein expression levels of CDK6,CDK4,Bax,Bcl-2,caspase-3,P65,and the PI3K/Akt/mTOR pathway in gastric cancer cell lines after curcumin treatment.Differential expression of lncRNAs before and after curcumin treatment was assessed using lncRNA sequencing and validated using quantitative reverse

关 键 词：Gastric cancer CURCUMIN Long noncoding RNA AC022424.2 Apoptosis Akt/PI3K pathway Lymph metastasis 

分 类 号：R735.34[医药卫生—肿瘤]