基于硼氢化钠原位还原的纳米金杂化酶的制备及催化阿魏酸甘油酯合成  

Synthesis of Glycerol Ferulate Catalysed by NaBH_(4)@AuNPs-CRL Hybridases Prepared by in situ Reduction of Sodium Borohydride

作　　者：尹一 孙立瑞 刘青云 辛嘉英[1,2] YIN Yi;SUN Li-rui;LIU Qing-yun;XIN Jia-ying(Key Laboratory for Food Science and Engineering,Harbin Uninversity of Commerce,Harbin 150076,China;State Key Laboratory for Oxo Synthesis&Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]哈尔滨商业大学食品科学与工程重点实验室,黑龙江哈尔滨150076 [2]中国科学院兰州物理化学研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《分子催化（中英文）》2024年第1期26-34,I0001,I0002,共11页Journal of Molecular Catalysis(China)

基　　金：中央支持地方高校改革发展资金人才培养支持计划项目(No.304017)。

摘　　要：建立了一种基于硼氢化钠原位还原法制备纳米金杂化酶用以合成阿魏酸甘油酯的方法,使杂化酶的催化活性、稳定性及结构的刚性皆有所提高.利用硼氢化钠原位还原法制备了NaBH_(4)@AuNPs-CRL杂化酶,通过单因素实验得到了最优的杂化条件,并利用荧光光谱、红外光谱、X-射线电子能谱、透射电镜等方法探究了杂化前后酶结构的变化.研究表明制备的NaBH_(4)@AuNPs-CRL杂化酶蛋白二级结构中α-螺旋含量减少、β-折叠含量增加,杂化酶比活性为4.91±0.12 U∙mg^(-1),与游离酶相比提高了66.44%,最佳条件下催化合成阿魏酸甘油酯的转化率为98.39%±3.65%,批式操作稳定性实验表明杂化酶的稳定性有较大提升.In this work,a method based on the in situ reduction of sodium borohydride for the preparation of gold nanobiohybrids for the synthesis of glycerol ferulate was established,which resulted in better activity,stability and structural rigidity of the hybridases.The optimum conditions for the preparation of the NaBH_(4)@AuNPs-CRL hybridases were obtained by a one-way experiment,and the changes of the lipase before and after hybridisation were explored using fluorescence spectroscopy,infrared spectroscopy,X-ray electron spectroscopy and transmission electron microscopy.It has been shown that the NaBH_(4)@AuNPs-CRL hybridases have a reduction ofα-helix and an increase ofβ-folding in their secondary structure.The specific activity of the NaBH_(4)@AuNPs-CRL hybridases was 4.91±0.12 U∙mg^(-1),which was 66.44%higher compared to CRL.The conversion of the glycerol ferulate synthesis catalysed by the NaBH_(4)@AuNPs-CRL hybridases under the optimum conditions was 98.39%±3.65%,and the stability experiments carried out in batch mode showed that both the activity and the stability had been improved.

关 键 词：脂肪酶 纳米金 硼氢化钠 杂化酶 阿魏酸甘油酯 

分 类 号：O643.32[理学—物理化学]