微生物组学方法评估介入超声引导下的前列腺癌活检对患者肠道菌群的影响  

Microbiomic approach to assess the impact of interventional ultrasound-guided prostate cancer biopsy on patients’intestinal flora

作　　者：李金 罗乐[2] 何糠 胡德军[2] 李明星 LI Jin;LUO Le;HE Kang;HU Dejun;LI Mingxing(The Affiliated Hospital of Southwest Medical University,Luzhou646000,Sichuan,China;People’s Hospital of Deyang City)

机构地区：[1]西南医科大学附属医院超声科,四川泸州646000 [2]德阳市人民医院超声科

出　　处：《中国病原生物学杂志》2024年第4期430-435,共6页Journal of Pathogen Biology

基　　金：四川省科学技术厅项目(No.14JC01873-LH27)。

摘　　要：目的评估介入超声引导下的前列腺活检(TPPBx)对患者肠道菌群结构的影响。方法本前瞻性队列研究选取2023年3月1日至10月30日在德阳市人民医院泌尿科就诊的15名因前列腺特异性抗原(PSA)升高建议行TPPBx的患者。采集患者TPPBx前后的粪便样本,并通过16S rRNA基因测序分析肠道微生物组成。结果放线菌门、拟杆菌门、厚壁菌门、变形菌门、疣微菌门和古菌门是肠道菌群的主要组成部分,占比超过80%。与Pre-TPPBx组相比,Post-TPPBx组中拟杆菌门的比例显著降低(16.20%±7.57%vs.13.12%±8.13%,t=2.583,P=0.013),而厚壁菌门的比例显著增加(14.29%±6.49%vs.16.51%±7.54%,t=2.962,P=0.029)。在多样性分析中,与Pre-TPPBx组相比,Post-TPPBx组患者的肠道菌群Alpha多样性指数有显著下降,包括Shannon指数(1.94±0.62 vs.1.54±0.54,t=-3.100,P=0.004)、Simpson指数(0.79±0.09 vs.0.55±0.09,t=4.751,P=0.001)和Chao1指数(31.51±11.36 vs.27.68±9.27,t=2.964,P=0.007)。Beta多样性分析也显示了两组之间明显的差异分布。LEfSe分析显示,Post-TPPBx样本中与缓解炎症相关的细菌属如Eubacterium eligens group、Lachnospira和Butyricicoccus的相对丰度更高,Pre-TPPBx样本中富集了多个与炎症和焦虑有关的细菌属。结论TPPBx可能会改变患者的肠道微生物群结构,具体表现为增加有益菌属和与减少炎症相关的菌属。本研究强调了在前列腺癌诊断中考虑微生物组的重要性,并为未来的研究提供了新的方向。Objective To evaluate the impact of interventional ultrasound-guided prostate biopsy(TPPBx)on the structure of patients’intestinal microbiota.Methods This prospective cohort study selected 15patients who were recommended to undergo TPPBx due to elevated prostate-specific antigen(PSA)in the Urology Department of Deyang People’s Hospital from March 1to October 30,2023.Fecal samples were collected from patients before and after TPPBx,and the intestinal microbial composition was analyzed by 16SrRNA gene sequencing.Results Actinobacteria,Bacteroidetes,Firmicutes,Proteobacteria,Verrucomicrobia and Archaea were the main components of the intestinal flora,accounting for more than 80%.Compared with the Pre-TPPBx group,the proportion of Bacteroidetes in the Post-TPPBx group was significantly reduced(16.20%±7.57%vs.13.12%±8.13%,t=2.583,P=0.013),while the proportion of Firmicutes was significantly increased(14.29%±6.49%vs.16.51%±7.54%,t=2.962,P=0.029).In the diversity analysis,compared with the Pre-TPPBx group,the intestinal flora Alpha diversity index of patients in the Post-TPPBx group was significantly reduced,including the Shannon index(1.94±0.62vs.1.54±0.54,t=-3.100,P=0.004),Simpson index(0.79±0.09vs.0.55±0.09,t=4.751,P=0.001)and Chao1index(31.51±11.36vs.27.68±9.27,t=2.964,P=0.007).Beta diversity analysis also showed clear differential distribution between the two groups.LEfSe analysis showed that the relative abundance of bacterial genera related to alleviating inflammation,such as Eubacterium eligens group,Lachnospira,and Butyricicoccus,was higher in Post-TPPBx samples,and multiple bacterial genera related to inflammation and anxiety were enriched in Pre-TPPBx samples.Conclusion TPPBx may change the intestinal microbiota structure of patients,specifically by increasing beneficial bacterial genera and bacterial genera related to reducing inflammation.This study highlights the importance of considering the microbiome in prostate cancer diagnosis and provides new directions for future research.

关 键 词：前列腺活检 肠道微生物组 16S rRNA基因测序 TPPBx 微生物群结构 

分 类 号：R737.25[医药卫生—肿瘤]