基于微生物发酵前处理的桑黄多糖提取工艺研究  

Study on Extraction Technology of Polysaccharides from PhellinusIgniarius Based on Pretreatment of Microbial Fermentation

作　　者：庞维妮 张洋[1] 费思瑜 王洪娇 董梓涵 刘赟霄 刘兵 李加友[1] PANG Wei-ni;ZHANG Yang;FEI Si-yu;WANG Hong-jiao;DONG Zi-han;LIU Yun-xiao;LIU Bing;LI Jia-you(College of Biological and Chemical Engineering,Jiaxing University,Zhejiang Jiaxing 314001;Agricultural and Rural Administration Bureau of Haidong City,Qinghai Haidong 810600,China)

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [2]青海省海东市农业农村局,青海海东810600

出　　处：《广州化工》2023年第22期51-53,共3页GuangZhou Chemical Industry

基　　金：青海省科技计划重点研发与转化计划(2019-NK-113);浙江省大学生科技创新活动计划(新苗人才计划)(2023R417007)。

摘　　要：以经过康宁木霉菌LZ51(Trichoderma koninggii)发酵处理得到的发酵桑黄为原料,总多糖提取率为指标,采用正交试验与单因素实验相结合的方法,考察浸提过程中提取剂用量、提取时间及提取温度对提取率的影响,确定最佳提取工艺。结果表明:以水为提取剂、用量为45 mL/g, 100℃下浸提2 h,总多糖提取率最高,为48.44 mg/g。而同批次未经发酵前处理的桑黄,在相同条件下浸提,总多糖提取率仅为29.36 mg/g,远低于发酵桑黄总多糖提取率。由此可见,微生物发酵前处理可显著提高桑黄总多糖提取率。With the Phellinus igniarius obtained by LZ51(Trichoderma koninggii)as the raw material,the total polysaccharide extraction rate as the inspection index,the orthogonal experiment and single factor experiment were used to investigate the effects of the amount of extracting agent,the extraction time and the extraction temperature on the extraction rate during the reflux extraction process.The results showed that the highest extraction rate of total polysaccharide was 48.44 mg/g when water was used as extraction agent,the dosage was 45 mL/g,and the extraction time was 2 h at 100℃.The total polysaccharide extraction rate of the same batch of Phellinus igniarius without fermentation treatment was only 29.36 mg/g.It could be seen that the pretreatment of microbial fermentation can significantly improve the extraction rate of total polysaccharide from Phellinus igniarius.

关 键 词：微生物发酵 前处理 桑黄 多糖 提取工艺 

分 类 号：R284.2[医药卫生—中药学]