青岛市胶州地区B(A)亚型患者的血型血清学及基因检测分析  

Blood group serology and gene detection in patients with B(A)subgroup in Jiaozhou,Qingdao

作　　者：秦洪伟 王小霞 阴瑞兰 张树超[1,3] QIN Hongwei;WANG Xiaoxia;YIN Ruilan;ZHANG Shuchao(Department of Immunology,College of Basic Medicine,Qingdao University,Qingdao 266071,China;Department of Blood Transfusion,Jiaozhou Central Hospital of Qingdao;Department of Blood Transfusion,the Affiliated Hospital of Qingdao University)

机构地区：[1]青岛大学基础医学院免疫学系,山东青岛266071 [2]青岛市胶州中心医院输血科 [3]青岛大学附属医院输血科

出　　处：《中国输血杂志》2024年第3期325-330,共6页Chinese Journal of Blood Transfusion

摘　　要：目的调查并分析青岛市胶州地区某三级医院就诊患者B(A)亚型的血清学及分子生物学特征。方法2019年11月至2023年2月,12名患者血液标本经微柱玻璃珠法和盐水试管法ABO血型检测疑为AB亚型,进一步对其ABO基因第6、7外显子进行直接扩增、测序和分析,确定ABO等位基因型别。结果青岛市胶州地区26065名患者中共检测发现9例B(A)亚型,检出率为0.345‰(9/26065);9例B(A)亚型中,8例血清学反应表现A_(w)B,基因检测为BA.04基因与O基因杂合,1例血清学反应表现为ABw,基因检测为BA.04基因与A基因杂合。结论血型血清学结合基因检测可准确鉴定ABO血型,在AB_(w)血清学反应的个体中,有可能存在B(A)亚型等位基因。Objective To investigate and analyze the serological and molecular biological characteristics of B(A)subgroup in a tertiary hospital in Jiaozhou,Qingdao.Methods From November 2019 to February 2023,the samples of 12 patients were suspected to be AB subgroup by microcolumn glass bead method and saline test tube method.The exons 6 and 7 of ABO gene were further amplified,sequenced and analyzed to determine the ABO allele type.Results A total of 9 cases of B(A)subgroup were detected in 26065 patients in Jiaozhou,with a detection rate of 0.345‰(9/26065).Among the 9 cases of B(A)subgroup,8 cases of serological reaction showed A weak B,and the gene detection was heterozygous for BA.04 gene and O gene.One case of serological reaction showed AB weak,and the gene detection was heterozygous for BA.04 gene and A gene.Conclusion Blood group serological combined with gene detection can accurately identify ABO blood group.B(A)subgroup alleles can exist in individuals with serological reaction of AB weak.

关 键 词：ABO血型 B(A)亚型 血型血清学 基因测序 青岛 

分 类 号：R457.1+1[医药卫生—治疗学]