辣木籽蛋白酶解物对小鼠肠道黏膜炎的改善作用  

Ameliorating Effects of Moringa oleifera Seed Protein Hydrolysate on Intestinal Mucositis in Mice

作　　者：杨晓丽 李丽 杨静娟 田洋[1,2] 白忠彬 YANG Xiaoli;LI Li;YANG Jingjuan;TIAN Yang;BAI Zhongbin(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;National Research and Development Professional Center for Moringa Oleifera Processing Technology,Kunming 650201,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]国家辣木加工技术研发专业中心,云南昆明650201 [3]云南农业大学动物医学院,云南昆明650201

出　　处：《现代食品科技》2024年第3期1-8,共8页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31960508);国家木薯产业技术体系“辣木产品加工岗位”(CARS-11-YNTY);云南绿色食品国际合作研究中心项目(省科技厅)(2019ZG00905);云南省重大科技专项计划(202002AA100005)。

摘　　要：探讨辣木籽蛋白酶解物(Moringa oleifera Seed Protease Hydrolysate,MSPH)对小鼠肠道黏膜炎的改善作用。提取辣木籽蛋白并进行酶解,冷冻干燥得到MSPH;随机将雄性BALB/c小鼠分为空白对照(Con)组、模型(Mod)组和MSPH组(800 mg/kg·BW),每组6只,腹腔注射5-FU诱导小鼠肠道黏膜炎,MSPH灌胃干预。每天记录小鼠体质量;通过HE染色观察肠道组织形态学并计算绒毛高度/隐窝深度;酶联免疫法测定小鼠血清(INF-γ、SIgA、ROS、G-CSF、ET、D-LA)和小肠组织(IL-1β、IL-6、MDA、MPO、TNF-α)含量变化;实时荧光定量聚合酶链式反应检测小肠组织ZO-1和Occludin m RNA表达量。与Mod组相比,MSPH组小鼠在实验后期体质量显著提高(P=0.001 1),脾脏和胸腺指数分别增加16.22%和34.69%,肠绒毛高度/隐窝深度增加25.88%,血清中INF-γ、ROS、G-CSF、ET、D-LA含量分别降低16.85%、17.50%、14.22%、8.28%、9.27%,SIgA含量升高23.07%,小肠组织中IL-1β、IL-6、MDA、MPO、TNF-α含量分别降低9.72%、11.23%、9.74%、7.29%、5.17%,肠道组织ZO-1和Occludin mRNA表达量增加121.64%和29.35%。因此,MSPH可以通过减轻炎症和氧化应激及降低肠道通透性改善5-FU诱导的小鼠肠道黏膜炎。The ameliorating effects of Moringa oleifera seed protein hydrolysate(MSPH)on intestinal mucositis in mice were investigated.Moringa oleifera seed protein was extracted,enzymolized,and freeze-dried to obtain MSPH.Male BALB/c mice were randomly divided into the blank control(Con)group,the model(Mod)group,and the MSPH treatment group with six mice in each group.5-fluorouracil(5-FU)was intraperitoneally injected to induce intestinal mucositis in mice,and MSPH(800 mg/kg·BW)was administered orally for intervention.The weights of the mice were recorded daily.The intestinal histopathology was observed using HE staining,and the villus height/crypt depth was calculated.The concentrations of INF-γ,SIgA,ROS,G-CSF,ET,and D-LA in serum and those of IL-1β,IL-6,MDA,MPO,and TNF-αin the small intestine were determined using enzyme-linked immunosorbent assay.The mRNA expressions of ZO-1 and occludin in the small intestine were determined using real-time fluorescence quantitative polymerase chain reaction.Compared to the mice in the Mod group,the body masses of those in the MSPH group significantly increased(P=0.0011)in the later stage of the experiment,while their spleen and thymus indices increased by 16.22%and 34.69%,respectively.The villus height/crypt depth increased by 25.88%in the MSPH group.The INF-γ,ROS,G-CSF,ET,and D-LA contents decreased by 16.85%,17.50%,14.22%,8.28%,and 9.27%,respectively,while the SIgA content increased by 23.07%in the MSPH group compared to that in the Mod group.The contents of IL-1β,IL-6,MDA,MPO,and TNF-αdecreased by 9.72%,11.23%,9.74%,7.29%,and 5.17%,respectively,while mRNA expressions of ZO-1 and occludin in intestinal tissues increased by 121.64%and 29.35%,respectively,in the MSPH group.Thus,5-FU-induced intestinal mucositis in mice can be ameliorated by MSPH intervention owing to reduction in inflammation,oxidative stress and intestinal permeability.

关 键 词：辣木籽蛋白酶解物 5-氟尿嘧啶 肠道黏膜炎 缓解改善 

分 类 号：TS201.4[轻工技术与工程—食品科学]