马齿苋中抗炎活性物质的提取、分离及结构鉴定  

Extraction,Separation and Structural Identification of Anti-inflammatory Active Substances from Purslane(Portulaca oleracea L.)

作　　者：张会敏 邢岩 仇润慷 张丽梅 倪贺[3] 赵雷[1] ZHANG Huimin;XING Yan;QIU Runkang;ZHAGN Limei;NI He;ZHAO Lei(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Guozhen Health Technology(Beijing)Co.Ltd.,Beijing 100000,China;College of Life Sciences,South China Normal University,Guangzhou 510640,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]国珍健康科技(北京)有限公司,北京100000 [3]华南师范大学生命科学学院,广东广州510640

出　　处：《现代食品科技》2024年第3期191-199,共9页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(31771980);广东省自然科学基金(2023A1515012599)。

摘　　要：以活性物质示踪为导向,建立脂多糖诱导的RAW264.7巨噬细胞炎症模型对马齿苋中的抗炎物质进行跟踪,采用柱层析提取法、硅胶柱色谱分离法、制备液相色谱法及气相色谱-质谱联用技术对抗炎物质进行提取分离和结构鉴定。结果表明,石油醚-乙醇、无水乙醇和纯水溶剂依次对马齿苋样品进行提取,三种粗提物将细胞中一氧化氮(Nitric Oxide,NO)的分泌量分别减少至33.13、25.83和20.53μmol/L,其中石油醚相粗提物的抑制效果最强(P<0.05)。对石油醚相进一步分离得到四个组分,Fr.1、Fr.2和Fr.3组分具有较强的抗炎效果,但Fr.1和Fr.2组分含有潜在的毒性成分,选择Fr.3组分继续分离。Fr.3组分经硅胶柱分离得到三个组分,Fr.3.1组分表现出最强的抑制NO的分泌量效果(11.80μmol/L)。经制备液相色谱进一步纯化及气质分析,确定Fr.3.1组分的主要成分为硬脂酸(47.09%)、邻苯二甲酸二(2-乙基己)酯(13.21%)和其他成分。该研究建立了一种从马齿苋中分离纯化出抗炎物质方法,为马齿苋的开发利用提供理论参考。To track the anti-inflammatory substances in purslane,the lipopolysaccharide-induced RAW264.7 macrophage inflammation model was established,which was guided by the tracer of active substances.The extraction,separation and structural identification of anti-inflammatory substances in purslane were performed by column chromatography(for extraction),silica gel column chromatography(for separation),and preparative high performance liquid chromatography and gas chromatography-mass spectrometry(for analyses).The results showed that the three crude extracts obtained from purslane through sequential extractions with petroleum ether-ethanol,anhydrous ethanol and pure water solvents reduced the secretion of nitric oxide(NO)in the cells to 33.13,25.83 and 20.53μmol/L,respectively,with the crude petroleum ether extract exhibiting the strongest inhibitory effect(P<0.05).The petroleum ether phase was further separated into four fractions,with the Fr.1,Fr.2 and Fr.3 fractions had stronger anti-inflammatory effects,though the Fr.1 and Fr.2 fractions contained potential toxic components.Therefore,the Fr.3 fraction was selected for further separation.The Fr.3 fraction was separated through a silica gel column to obtain three fractions.The Fr.3.1 subfraction exhibited the strongest inhibitory effect against the NO secretion(11.80μmol/L).The Fr.3.1 subfraction was further purified by the preparative liquid chromatography and GC-MS analysis,and the main components of the Fr.3.1 subfraction were identified as stearic acid(47.09%),di(2-ethylhexyl)phthalate(13.21%)and other components.This study established a method for separating and purifying anti-inflammatory substances from purslane,and provides a theoretical reference for the development and utilization of purslane.

关 键 词：马齿苋 抗炎活性 提取分离 鉴定 

分 类 号：TS201.2[轻工技术与工程—食品科学]