坛紫菜叶状体颜色与长度、宽度和厚度的相关性分析  

Correlation analysis of blade color with length,width and thickness in Pyropia haitanensis

作　　者：裘叶帆 丁洪昌 唐隆晨 严兴洪[1,2] QIU Yefan;DING Hongchang;TANG Longchen;YAN Xinghong(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [2]上海海洋大学上海水产养殖工程技术研究中心,上海201306

出　　处：《上海海洋大学学报》2024年第2期371-379,共9页Journal of Shanghai Ocean University

基　　金：国家自然科学基金青年基金(32102773);上海市青年科技英才扬帆计(21YF1417100);宁波市公益性科技计划项目(2022S160)。

摘　　要：实验以坛紫菜野生型品系(W,♀)和红色突变体(R,♂)杂交产生的杂合丝状体为材料,构建由240个株系组成的双单倍体(Doubled haploid,DH)群体,随后对DH群体的叶状体颜色与长度、宽度、叶片厚度、原生质体大小和细胞壁厚度5个数量性状的相关性进行分析。结果显示,在不同颜色组中(野生色组和红色组),5个性状的表型值均呈正态分布。各性状的变异系数为10.02%~43.73%,其中长度的变异系数最大。不同颜色组间,每个性状表型值呈极显著差异(P<0.01),相比野生色组,红色组的长度更长、宽度更窄、叶片更薄、原生质更小、细胞壁更薄。其中颜色与各性状的相关性程度:叶片厚度>细胞壁厚度>原生质体大小>长度>宽度。另外,各性状的遗传力均表现为长度>原生质体大小>叶片厚度>细胞壁厚度>宽度,且野生色组高于红色组。不同颜色组控制同一经济性状的基因对数不同,且控制长度性状的基因对数差异最大。本研究为开展坛紫菜复合经济性状新品种培育提供参考。A doubled haploid(DH)population consisting of 240 strains of Pyropia haitanensis was constructed using the heterozygous filament derived from the cross between the wild-type strain(W,♀)and the red mutant(R,♂)in the experiment.The correlation of blade color with the five quantitative traits of length,width,blade thickness,protoplast size and cell wall thickness of gametophytic blades was subsequently analyzed.The results showed that the phenotypic values of the five traits were normally distributed in different color groups(the wild-type group and the red-type group).The variation coefficient of each trait ranged from 10.02%to 43.73%,and the variation coefficient of length was the largest.The phenotypic values of each trait showed significant differences among different color groups(P<0.01).Compared with the wild-type group,the blades of red-type group had longer length,narrower width,thinner blade,smaller protoplast and thinner cell wall.The correlation degrees between color and each trait were ranked in the order of blade thickness>cell wall thickness>protoplast size>length>width.In addition,the heritability order of each trait was presented as length>protoplast size>blade thickness>cell wall thickness>width,and the heritability in the wild-type group was higher than that in the red-type group.The genes controlling the same economic trait were different in different color groups,and the genes controlling the length trait were the most different.This study provides reference for developing new varieties with compound economic traits in P.haitanensis.

关 键 词：坛紫菜 DH群体 数量性状 遗传分析 

分 类 号：S917.3[农业科学—水产科学]