致土腥味链霉菌的拮抗菌筛选、鉴定及抑制作用  

Screening,identification and inhibition of antagonistic bacteria against offflavor-producing Streptomyces

作　　者：徐岩 李慷[1,2,3] 刘晟 纪霜 罗雪能 刘利平[1,2,3] XU Yan;LI Kang;LIU Sheng;JI Shuang;LUO Xueneng;LIU Liping(China-ASEAN“The Belt and Road”Joint Laboratory of Marine Culture Technology(Shanghai),Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploitation and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学中国-东盟海水养殖技术“一带一路”联合实验室(上海),上海201306 [2]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [3]上海海洋大学上海水产养殖工程技术研究中心,上海201306

出　　处：《上海海洋大学学报》2024年第2期380-388,共9页Journal of Shanghai Ocean University

基　　金：上海市科技兴农技术创新项目(沪农科创字〔2021〕第3-1号);国家重点研发计划“蓝色粮仓科技创新”重点专项(2019YFD0900303)。

摘　　要：为筛选可抑制水产养殖中产土臭素(GSM)和2-甲基异莰醇(2-MIB)的链霉菌生长能力的益生菌,以嗜热一氧化碳链霉菌、蓝微褐链霉菌和玫瑰黄链霉菌为指示菌株,进行抑菌活性筛选,并对具有抑菌活性菌株进行鉴定。共筛选获得8株拮抗菌,结果表明其中2株为地衣芽孢杆菌,6株为枯草芽孢杆菌。所筛菌株对供试链霉菌的生长均有抑制作用,其中2株可抑制嗜热一氧化碳链霉菌和玫瑰黄链霉菌的生长,另外6株对嗜热一氧化碳链霉的生长有抑制作用。筛选出的8株细菌可作为防控土腥味的益生菌,为生物控制和去除水产养殖中的土腥味提供了理论参考和实践依据。In order to screen probiotics that can inhibit the growth of Streptomycetes spp.that produces geosmin(GSM)and 2-methylisobcamol(2-MIB)in aquaculture,Streptomyces thermocarboxydus,S.cyaneofuscatus and S.roseoflavus were used as test strains to carry out inhibition experiments,and the screened strains were identified.The results showed that eight strains of probiotics had inhibitory effects on the growth of S.spp.,2 strains of them could inhibit the growth of S.thermocarboxydus and S.roseoflavus,meanwhile,the other 6 strains affected the growth of S.thermocarboxydus.2 strains belonged to Bacillus licheniformis and 6 strains belonged to B.subtilis.The 8 screened strains can serve as probiotics for preventing and controlling off-flavor,providing theoretical reference and practical basis for biological control and removal of off-flavor in aquaculture.

关 键 词：土臭素 2-甲基异莰醇 拮抗菌 筛选 鉴定 

分 类 号：X172[环境科学与工程—环境科学]