表面硫化普鲁士蓝比色法测定GSH的综合实验设计  

Experiment Design for Colorimetric Determination of GSH Based on Surface Vulcanization Prussian Blue

作　　者：徐丹 王璐璐 江家慧 许育森 李海霞[1] XU Dan;WANG Lulu;JIANG Jiahui;XU Yusen;LI Haixia(School of Pharmacy,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医学院药学院,海口571199

出　　处：《实验室研究与探索》2024年第2期198-202,248,共6页Research and Exploration In Laboratory

基　　金：海南医学院校级教育科研课题(HYYB202160);海南省高等学校教育教学改革重点项目(Hnjg2023ZD-35)。

摘　　要：设计了以硫化法制备表面硫化的普鲁士蓝催化剂,用于比色法检测谷胱甘肽(GSH)。该催化剂可以有效消除本身蓝色的干扰,显著提升类过氧化物酶活性。在H_(2)O_(2)存在下,该催化剂催化3,3′,5,5′-四甲基联苯胺(TMB)氧化形成蓝色的氧化型TMB(oxTMB)。动力学分析表明,催化剂与底物的亲和力强,呈现优异的类过氧化物酶活性。硫化的普鲁士蓝用于比色法检测GSH时表现出较高的灵敏度和选择性。实验包含材料制备、仪器表征和检测,可分割成多个模块在无机化学、仪器分析和生物化学中开展,有利于增强学生创新实践能力,提高学生整合所学知识的能力。In this experiment,the application of Prussian blue in biological analysis is studied.Based on the UV-Vis spectrophotometry method,combining the knowledge of chemistry and biochemistry,a surface vulcanization Prussian blue catalyst is innovatively prepared by sulfide method.The catalyst can effectively eliminate the interference of its own blue color and significantly improve the activity of peroxidase.In the presence of H_(2)O_(2),the catalyst catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine(TMB)to form blue oxidized TMB(oxTMB).Kinetic analysis shows that the catalysts have strong affinity with substrates and present excellent peroxidase activity.The surface vulcanization Prussian blue exhibits high sensitivity and selectivity in the detection of glutathione by colorimetry.This experiment including material preparation,instrument characterization and detection,can be divided into multiple modules to carry out experimental teaching in inorganic chemistry,instrumental analysis and biochemistry.It is conducive to enhancing innovative and practical ability of students,and improving the ability of integrating knowledge.

关 键 词：普鲁士蓝 表面硫化 类过氧化物酶活性 比色法 实验教学 

分 类 号：O614.8[理学—无机化学] G642.423[理学—化学]