响应面试验优化福林酚法测定三叶木通果皮多酚含量及其抗氧化活性  

Optimization of the Polyphenol Content of the Peel of Akebia trifolia by Folin-ciocalteu Method of Response Surface Methodology and its Antioxidant Activity

作　　者：李艳辉 杨蕊西 何靖怡 袁亚宏 岳田利 盛庆林 LI Yanhui;YANG Ruixi;HE Jingyi;YUAN Yahong;YUE Tianli;SHENG Qinglin(College of Food Science and Technology,Northwest University,Xi'an,Shaanxi 710069,China)

机构地区：[1]西北大学食品科学与工程学院,陕西西安710069

出　　处：《农产品加工》2024年第5期70-74,共5页Farm Products Processing

基　　金：国家重点研发计划项目课题(2019YFC1606703)。

摘　　要：对比80%甲醇、60%乙醇、水3种溶剂提取的三叶木通果皮的体外抗氧化活性。结果表明,80%甲醇提取物具有最强的总抗氧化能力(20.51±0.02) mg/g,清除DPPH自由基能力(28.33±0.12) mg/m L,对Fe^(2+)络合能力(23.11±0.14) mg EDTA-2Na/g,对·OH引起的p BR322质粒DNA氧化损伤的保护能力。采用响应面优化福林酚法测定三叶木通果皮80%甲醇粗提粉的多酚含量,将福林酚稀释倍数设为2,将20%碳酸钠用量设为1 m L,将反应温度设定为27℃,将反应时间设定为110 min,所得80%甲醇提取物中多酚含量为(18.91±0.38) mg/g。By comparing the in vitro antioxidant activities of three solvent extracts of Akebia trifolia in 80%methanol,60%ethanol and water,the results showed that the 80%methanol extract had the highest total antioxidant capacity(20.51±0.02)mg/g,DPPH scavenging capacity(28.33±0.12)mg/mL,ability to complex Fe^(2+)(23.11±0.14)mg EDTA-2Na/g and protection against·OH-induced oxidative damage to pBR322 plasmid DNA.Then the response surface optimization Folin-ciocalteu Method was used to determine the polyphenol content of 80%methanol crude extract powder from the pericarp of Akebia trifolia,the dilution of Folin-ciocalteu reagent was set to 2,the dosage of 20%sodium carbonate was 1mL,the reaction temperature was 27℃,the reaction time was 110 min,and the polyphenol content from the 80%methanolic extract obtained was(18.91±0.38)mg/g.

关 键 词：三叶木通 八月瓜多酚 体外抗氧化能力 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]