胆巴浸泡附子的工艺研究  

Process of Soaking Fuzi in Solution of Danba

作　　者：郑琪 何成军 刘杰 耿昭 邱波 蒙春旺 周勤梅[1,4] Zheng Qi;He Chengjun;Liu Jie;Geng Zhao;Qiu Bo;Meng Chunwang;Zhou Qinmei(Systematic Research,Development and Utilization of Traditional Chinese Medicine Resources in School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;Sichuan Institute of Drug Inspection,Sichuan Medical Device Testing Center,Key Laboratory of Quality Evaluation of Proprietary Chinese Medicine,Chengdu,611731,China;Sichuan Jinzhuobang Chemical Technology Co.,Ltd.,Suining,6293083,China;Institute of Traditional Chinese Medicine Innovation,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China)

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室,四川成都611137 [2]四川省药品检验研究院四川省医疗器械检测中心国家药品监督管理局中成药质量评价重点实验室,四川成都611731 [3]四川金卓筒化工科技股份有限公司,四川遂宁629308 [4]成都中医药大学中医药创新研究院,四川成都611137

出　　处：《成都中医药大学学报》2024年第2期18-22,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82104546)。

摘　　要：目的:探究附子炮制过程中胆巴浸泡工艺对附子生物碱的影响,确定最佳的工艺参数。方法:从胆巴水溶液的浓度、附子在胆巴水溶液中的浸泡时间、附子与胆巴水溶液的料液比三个方面进行单因素考察,参照《中国药典》2020年版附子的含量测定方法,采用高效液相色谱法对附子中的生物碱含量进行测定,分析附子中生物碱的含量变化。结果:附片中双酯型生物碱含量随着泡胆时间增加、胆巴水溶液料液比减小和胆巴水溶液浓度增大,总体呈下降趋势;单酯型生物碱的含量变化无明显规律;总生物碱含量均呈下降趋势。综合附子的形态和生物碱含量,确定胆巴浸泡的最佳工艺为35%-40%胆巴水溶液浓度,以1∶2的料液比浸泡附子不少于20 d。结论:胆巴浸泡能有效保存附子,并能够降低其有毒成分双酯型生物碱的含量,优选的浸泡工艺可为胆巴炮制附子规范提供参考。Objective:To explore the effect of Danba soaking process on aconite alkaloids in the processing of Fuzi,and determine the best process parameters.Methods:A single factor investigation was carried out from the three aspects of the concentration of the Danba aqueous solution,the soaking time of the Fuzi in the Danba aqueous solution,and the solid-liquid ratio of the aconite and the Danba aqueous solution.The HPLC method was used to determine the content of the Fuzi according to the "Chinese Pharmacopoeia" 2020 edition.The alkaloid content in the aconite was determined,and the change of the alkaloid content in the aconite was analyzed.Results:The content of diester alkaloids in Fuzi showed a general downward trend with the prolongation of bile infusion time,the decrease of the solid-liquid ratio of bile solution and the increase of the concentration of bile solution;the content of monoester alkaloids had no obvious change;The total alkaloid content showed a downward trend.Based on the morphology and alkaloid content of Fuzi,the best process for soaking Danba was determined to be 35%-40% Danba aqueous solution concentration,and the aconite soaked at a solid-liquid ratio of 1∶2 for no less than 20 days.Conclusion:Danba soaking can effectively preserve Fuzi and reduce the content of its toxic components,diester-type alkaloids.The optimal soaking process can provide a reference for the processing of Fuzi in Danba.

关 键 词：附子 胆巴 浸泡工艺 生物碱 含量测定 

分 类 号：R283.3[医药卫生—中药学] R[医药卫生—中医学]