Way to efficient microbial paclitaxel mass production  

作　　者：Chenyue Li Yanli Qi Zhongke Sun Mengwan Jiang Chengwei Li 

机构地区：[1]School of Biological Engineering,Henan University of Technology,Zhengzhou,450001,China [2]Nanyang Institute of Medical Plant Technology and Industry,Nanyang,473005,China [3]School of Artificial Intelligence and Big Data,Henan University of Technology,Zhengzhou,450001,China

出　　处：《Synthetic and Systems Biotechnology》2023年第4期673-681,共9页合成和系统生物技术（英文）

基　　金：supported by a cooperative grant from Henan University of Technology(No.51100014);a grant from the Agency of Science and Technology of Henan Province(No.232102311153,No.221100110700).

摘　　要：The microbial synthesis of paclitaxel is attractive for its short-cycle,cost-effectiveness,and sustainability.However,low paclitaxel productivity,depleted capacity during subculture and storage,and unclear biosynthesis mechanisms restrain industrial microbial synthesis.Along with the isolation of various paclitaxel-producing microorganisms and the development of versatile molecular tools,tremendous promises for microbial paclitaxel synthesis have become increasingly prominent.In this review,we summarize the progress of microbial synthesis of paclitaxel in recent years,focusing on paclitaxel-producing endophytes and representative engineering microorganism hosts that were used as chassis for paclitaxel precursor synthesis.Numerous wide-type microbes can manufacture paclitaxel,and fermentation process optimization and strain improvement can greatly enhance the productivity.Engineered microbes can efficiently synthesize precursors of paclitaxel by introducing exogenous synthetic pathway.Mining paclitaxel synthetic pathways and genetic manipulation of endophytes will accelerate the construction of microbial cell factories,indefinitely contributing to paclitaxel mass production by microbes.This review emphasizes the potential and provides solutions for efficient microbial paclitaxel mass production.

关 键 词：PACLITAXEL Microbial fermentation ENDOPHYTES Process optimization Synthetic pathway 

分 类 号：Q939.9[生物学—微生物学]