Dietary choline activates the Ampk/Srebp signaling pathway and decreases lipid levels in Pacific white shrimp(Litopenaeus vannamei)  

作　　者：Jingjing Lu Xinyue Tao Jiaxiang Luo Tingting Zhu Lefei Jiao Peng Sun Qicun Zhou Douglas R.Tocher Min Jin 

机构地区：[1]Laboratory of Fish and Shellfish Nutrition,School of Marine Sciences,Ningbo University,Ningbo 315211,China [2]Guangdong Provincial Key Laboratory of Marine Biotechnology,Institute of Marine Sciences,Shantou University,Shantou 515063,China

出　　处：《Animal Nutrition》2023年第4期58-70,共13页动物营养（英文版）

基　　金：supported by the Natural Science Foundation of Zhejiang Province(LY21C190006,LY17C190002);the National Key R&D Program of China(2018YFD0900400);China Agriculture Research System-48(Supported by China Agriculture Research System of MOF and MARA);National Natural Science Foundation of China(32072987);the K.C.Wong Magna Fund of Ningbo University,China.

摘　　要：An 8-week feeding trial was conducted in Pacific white shrimp(Litopenaeus vannamei)to evaluate the effects of dietary choline supplementation on choline transport and metabolism,hepatopancreas histological structure and fatty acid profile,and regulation of lipid metabolism.Six isonitrogenous and isolipidic diets were formulated to contain different choline levels of 2.91(basal diet),3.85,4.67,6.55,10.70 and 18.90 g/kg,respectively.A total of 960 shrimp(initial weight,1.38±0.01 g)were distributed randomly into twenty-four 250-L cylindrical fiber-glass tanks,with each diet assigned randomly to 4replicate tanks.The results indicated that dietary choline significantly promoted the deposition of choline,betaine and carnitine(P<0.05).The diameters and areas of R cells,total lipid and triglyceride contents in hepatopancreas,and triglyceride and non-esterified fatty acid contents in hemolymph were negatively correlated with dietary choline level.The contents of functional fatty acids in hepatopancreas,the activity of acetyl-CoA carboxylase(Acc),and the mRNA expression of fas,srebp and acc were highest in shrimp fed the diet containing 4.67 g/kg choline,and significantly higher than those fed the diet containing 2.91 g/kg,the lowest level of choline(P<0.05).The number of R cells,content of very lowdensity lipoprotein(VLDL),activities of carnitine palmitoyl-transferase(Cpt1),lipoprotein lipase and hepatic lipase,and the mRNA expression levels of cpt1,fabp,fatp,ldlr,and ampk in hepatopancreas increased significantly as dietary choline increased(P<0.05).In addition,hepatopancreas m RNA expression levels of ctl1,ctl2,oct1,badh,bhmt,ck,cept,and cct were generally up-regulated as dietary choline level increased(P<0.01).In conclusion,dietary choline promoted the deposition of choline and its metabolites by up-regulating genes related to choline transport and metabolism.Moreover,appropriate dietary choline level promoted the development of hepatopancreas R cells and maintained the normal accumulation of lipids required for devel

关 键 词：Pacific white shrimp CHOLINE Lipid Fatty acid Histology Ampk signaling pathway 

分 类 号：S968.22[农业科学—水产养殖]