Uptake and Intracellular Fate of Fluorophore Labeled Metal−Organic-Framework(MOF)Nanoparticles  

作　　者：Ziyao Liu Andreas Zimpel Ulrich Lächelt Maria Pozzi Marta Gallego Gonzalez Indranath Chakraborty Stefan Wuttke Neus Feliu Wolfgang J.Parak 

机构地区：[1]Center for Hybrid Nanostructures,Universität Hamburg,22761 Hamburg,Germany [2]Key Laboratory of Biological Nanotechnology of National Health Commission,Xiangya Hospital,Central South University,Changsha 410008,China [3]Department of Chemistry and Center for NanoScience(CeNS),LMU Munich,81377 Munich,Germany [4]Department of Pharmacy and Center for NanoScience(CeNS),LMU Munich,81377 Munich,Germany [5]Department of Pharmaceutical Sciences,University of Vienna,1090 Vienna,Austria [6]Center for Cooperative Research in Biomaterials(CIC BiomaGUNE),Basque Research and Technology Alliance(BRTA),20014 Donostia-San Sebastián,Spain [7]School of Nano Science and Technology,Indian Institute of Technology Kharagpur,Kharagpur 721302,India [8]BCMaterials,Basque Center for Materials,Applications and Nanostructures,48950 Leioa,Spain [9]IKERBASQUE,Basque Foundation for Science,48009 Bilbao,Spain

出　　处：《Environment & Health》2023年第4期270-277,共8页环境与健康（英文）

基　　金：supported by the project HeatNMof(European Union’s Horizon 2020 program).N.F.was funded by Fraunhofer Attract(Fraunhofer-Gesellschaft).Z.L.was supported by China Scholarship Council(CSC).

摘　　要：The uptake and the fate of Zr-based metal−organic-framework nanoparticles labeled with organic fluorophores in HeLa cells has been monitored with fluorescence detection and elemental analysis.The nanoparticles have been selected as a model system of carrier nanoparticles(here Zr-based metal−organic-framework nanoparticles)with integrated cargo molecules(here organic fluorophores),with aze that does not allow for efficient exocytosis,a material which only partly degrades under acidic conditions as present in endosomes/lysosomes,and with limited colloidal stability.Data show that,for Zr-based metal−organic-framework nanoparticles of 40 nm size as investigated here,the number of nanoparticles per cells decreases faster due to particle redistribution upon proliferation than due to nanoparticle exocytosis and that,thus,also for this system,exocytosis is not an efficient pathway for clearance of the nanoparticles from the cells.

关 键 词：nanoparticle endocytosis EXOCYTOSIS proliferation fate of nanoparticles intracellular degradation metal organic framework nanoparticles 

分 类 号：TB383[一般工业技术—材料科学与工程]