AITC调控肠嗜铬细胞中血清素的代谢及其生物学功能  

AITC regulates serotonin secretion and other biological functions in enterochromaffin cells

作　　者：王思瑜 杨雅丽 司琪琦 郭泰林[2] 黄新河[1] WANG Siyu;YANG Yali;SI Qiqi;GUO Tailin;HUANG Xinhe(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;College of Medicine,Southwest Jiaotong University,Chengdu 610031,China)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]西南交通大学医学院,成都610031

出　　处：《生物学杂志》2024年第2期51-56,共6页Journal of Biology

基　　金：四川省自然科学基金面上项目(No.23NSFSC0920)。

摘　　要：为探讨异硫氰酸烯丙酯(Allylisothiocyanate,AITC)调控肠嗜铬细胞(Enterochromaffin cells,EC)中血清素(5-hydroxytryptamine,5-HT)的合成及其对EC生物学功能的影响,使用食用级AITC对RIN-14B细胞(大鼠胰腺内分泌细胞系)进行干预处理,采用FLUO-8 AM检测RIN-14B细胞内钙离子浓度,qRT-PCR分析5-HT合成相关基因表达,UPLC测定5-HT含量,以及RNA-seq检测AITC处理后的RIN-14B细胞转录组,并对其结果进行生物信息学分析。结果表明,AITC通过激活离子通道TRPA1使胞内钙离子浓度升高,上调Tph1(色氨酸羟化酶)和Ddc(5-羟色氨酸脱羧酶)的表达,从而促进5-HT合成及分泌。此外,GO和KEGG功能富集分析AITC处理后的RIN-14B细胞差异表达基因发现,AITC主要调控谷胱甘肽代谢和抗氧化、炎症调节等相关通路,提示AITC可能通过刺激EC促进谷胱甘肽代谢来调节肠道稳态,同时参与肠道的炎症调节。以上结果为研究AITC影响EC及肠道更深入的生物学功能提供实验数据与研究思路。In order to investigate the effects of allyl isothiocyanate(AITC)on the synthesis of 5-HT in EC and the biological functions of EC,edible grade AITC were used to intervene with RIN-14B cells(rat pancreatic endocrine cell line),a cell model of EC.The calcium ion concentration in the cells was detected by FLUO-8 AM,the expression level of genes related to 5-HT synthesis was analyzed by qRT-PCR,the levels of 5-HT were determined by UPLC,and the transcriptome of AITC-treated RIN-14B cells was detected and analyzed by RNA-seq and bioinformatics.The results indicated that AITC caused an increase in intracellular calcium ion concentration through activation of TRPA1,while upregulated the expression of Tph1(tryptophan hydroxylase)and Ddc(5-hydroxytryptophan decarboxylase).In addition,GO and KEGG functional enrichment analysis on AITC treated RIN-14B cells showed that AITC mainly regulated glutathione metabolism and relate pathways such as antioxidant and inflammatory regulation,suggesting that AITC may regulate intestinal homeostasis by stimulating EC to promote glutathione metabolism,and also participate in inflammatory regulation of the intestine.The above results provided experimental data and research ideas for further study of the effects of AITC on EC and deeper biological functions of the intestine.

关 键 词：异硫氰酸烯丙酯 肠嗜铬细胞 血清素 RNA-SEQ 生物学功能 

分 类 号：Q78[生物学—分子生物学]