RCN1和GRP78在结直肠癌中的表达及临床意义  

Expression and clinical significance of RCN1 and GRP78 in colorectal cancer

作　　者：李灵玉 雷程[1] 王海江[1] LI Lingyu;LEI Cheng;WANG Haijiang(Affifiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属肿瘤医院,新疆乌鲁木齐830000

出　　处：《海南医学院学报》2024年第7期526-532,544,共8页Journal of Hainan Medical University

基　　金：新疆少数民族科技人才特殊培养计划科研项目(2021D03013)。

摘　　要：目的:检测RCN1和GRP78在结直肠癌组织中的表达水平,分析与临床病理特点的相关性,并探索预后价值。方法:采用TCGA及UALCAN数据库分析RCN1和HSPA5 mRNA在结直肠癌中的表达情况,收集临床标本进行免疫组化验证两者表达情况,分析与临床病理特征的相关性及预后价值。结果:生物信息学分析表明,RCN1和HSPA5 mRNA在结直肠癌中都显著高表达,并且两者表达呈正相关。RCN1表达与N分期及TP53突变显著相关,HSPA5在黏液腺癌中的表达较高。在结直肠癌组织中,RCN1和GRP78蛋白均高表达并定位于细胞质,GRP78的表达与T分期显著相关。Kaplan-Meier生存分析曲线显示,RCN1高表达组患者DFS明显低于低表达组患者,且有较短的OS趋势。多因素COX回归分析表明,神经侵袭、TNM分期及高表达的RCN1是结直肠癌DFS的独立危险因素。相关性分析:RCN1和GRP78在结直肠癌中表达呈正相关。结论:RCN1与GRP78表达呈正相关,是结直肠癌不良预后的独立危险因素。Objective:To investigate the relation of clinicopathological characteristics and RCN1 and GRP78 expression levels in colorectal cancer,and explore their prognostic value.Methods:The expression of RCN1 and HSPA5 mRNA in colorectal can-cer(CRC)was examined using the TCGA and UALCAN databases,and clinical samples were obtained for immunohistochemis-try to confirm the expression of both.Then,the correlation with clinicopathological features and its prognostic value were ana-lyzed.Results:According to a bioinformatics analysis,RCN1 and HSPA5 mRNA were both significantly overexpressed in CRC and had a positive correlation.The expression of RCN1 was correlated with N stage and TP53 mutations,the expression of HS-PA5 was higher in mucinous adenocarcinomas.In CRC tissues,RCN1 and GRP78 protein were both strongly expressed and local-ized in the cytoplasm.GRP78 expression was significantly correlated with T stage.The Kaplan-Meier survival analysis curve indi-cates that patients with higher RCN1 expression had significantly shorter DFS than those with lower expression and a tendency to-ward shorter OS.According to COX regression analysis,DFS in CRC was found to be independently associated with tumor nerve invasion,TNM stage,and high RCN1 expression,Correlation analysis:RCN1 and GRP78 expression were positively associated in CRC.Conclusion:RCN1 is an independent risk factor for a poor prognosis in colorectal cancer(CRC)and has a positive corre-lation with GRP78 expression.

关 键 词：RCN1 GRP78 结直肠癌 临床病理特征 预后 

分 类 号：R735.3[医药卫生—肿瘤]