乳酸菌作为口服黏膜免疫疫苗载体的改造方法  

Modification of Lactic Acid Bacteria as Vector of Oral Mucosal Immune Vaccine

作　　者：于天欣 孙钰[1,2,3,4] 石春卫 王春凤 曾艳[1,2,3,4] 曹欣 YU Tianxin;SUN Yu;SHI hunwei;WANG Chunfeng;ZENG Yan;CAO Xin(College of Veterinary Medicine,Jilin Agricultural University,Changchun 130118,China;Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding,Changchun 130118,China;Jilin Provincial Engineering Research Center of Animal Microecological Preparations,Changchun 130118,China;Key Laboratory of Animal Production and Product Quality and Safety of Ministry of Education,Changchun 130118,China)

机构地区：[1]吉林农业大学动物医学院,长春130118 [2]吉林省动物微生态与养殖重点实验室,长春130118 [3]吉林省动物微生态制剂工程研究中心,长春130118 [4]动物生产及产品质量安全教育部重点实验室,长春130118

出　　处：《经济动物学报》2024年第1期63-67,共5页Journal of Economic Animal

基　　金：长春市科技计划项目(21ZY42);吉林省科技发展计划项目(20200402041NC);国家自然科学基金项目(32273043,32202890,U21A20261);国家现代农业产业技术体系建设项目(CARS-35)。

摘　　要：乳酸菌是人和动物消化道内的重要益生菌,具有佐剂效应和免疫调节功能,以乳酸菌作为活疫苗载体表达外源蛋白,研制口服疫苗,具有重要意义和广阔的应用前景。对利用CRISPR/Cas9系统进行乳酸菌基因编辑、乳酸菌的锚定表达系统、表达FnBPA的侵入型乳酸菌、DCs靶向型乳酸菌和无抗筛选营养缺陷型乳酸菌等乳酸菌改造方法进行介绍,为乳酸菌载体疫苗的研制提供参考,并对其应用前景进行分析和展望。Lactic acid bacteria are important probiotics in the digestive tract of human and animals,and have adjuvant effect and immune regulation function.Therefore,it is of great significance and broad application prospect to use lactic acid bacteria as live vaccine vector to express foreign proteins to develop oral vaccines.In this paper,we mainly introduce the transformation methods of using CRISPR/Cas9 system for gene editing of lactic acid bacteria,anchored expression system of lactic acid bacteria,invasive lactic acid bacteria expressing FnBPA,DCS targeted lactic acid bacteria and non resistant screening lactic acid bacteria as oral mucosal immune vaccine vectors in order to provide reference for the development of lactic acid bacteria vector vaccine,and analyze and prospect its application prospects.

关 键 词：乳酸菌 黏膜免疫 口服疫苗 疫苗载体 改造方法 免疫保护 

分 类 号：S859.797[农业科学—临床兽医学]