载二甲双胍中空介孔硅纳米颗粒复合PLGA静电纺丝膜的制备、表征及体外生物学性能评价  

Preparation,characterization,and in vitro biological performance evaluation of PLGA electrospinning membranes doped with metformin-loaded hollow mesoporous silica nanoparticles

作　　者：靳瑞 王嘉 孙易初 李文硕 陈发明 安莹 JIN Rui;WANG Jia;SUN Yichu;LI Wenshuo;CHEN Faming;AN Ying(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi International Joint Research Center for Oral Diseases,Department of Periodontology,The Third Hospital Affiliated of Air Force Military Medical University,Xi'an,710032,China;16th Brigade,4nd Brigade of Cadets,School of Basic Medical Sciences,Air Force Medical University,Xi'an)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学第三附属医院牙周病科,西安710032 [2]空军军医大学基础医学院学员四大队十六队

出　　处：《实用口腔医学杂志》2024年第2期180-186,共7页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:82101049);陕西省重点研发计划(编号:2023-YBSF-007);陕西省青年科技新星(编号:2022KJXX-100)。

摘　　要：目的:制备载二甲双胍(Met)中空介孔硅纳米颗粒(HMSN)复合聚乳酸-羟基乙酸共聚物(PLGA)静电纺丝膜并研究其生物学性能。方法:采用静电纺丝技术制备PLGA(对照组)和PLGA/HMSN/Met电纺膜(实验组)。SEM观察两组电纺膜的微观形貌,同时检测亲疏水性、元素组成和体外药物释放。SEM、激光扫描共聚焦显微镜(LSCM)观察牙周膜干细胞(PDLSCs)在两组电纺膜上的生长情况,CCK-8法检测细胞增殖情况。结果:SEM结果显示两组电纺膜均具有类细胞外基质(ECM)纤维骨架结构,PLGA/HMSN/Met电纺膜缓释二甲双胍可达35 d,而且随着HMSN-Met的掺入,PLGA膜疏水性得到改善。SEM、活死细胞染色和细胞骨架染色结果表明复合电纺膜具有良好的体外生物相容性,CCK-8结果表明复合电纺膜可促进细胞增殖。结论:利用HMSN-Met对PLGA进行改性处理,可改善PLGA电纺膜疏水性、持续缓释二甲双胍,同时具有良好的细胞生物相容性。Objective:To prepare PLGA electrospinning membranes doped with hollow mesoporous silica nanoparticles loaded with metformin and investigate their biological properties.Methods:PLGA(Control group)and PLGA/HMSN/Met electrospun membranes(Experimental group)were prepared by electrospinning technology.The microscopic morphology of the 2 groups of electrospun membranes was observed by SEM.The hydrophilicity,elemental composition and in vitro drug release were detected by contact angle measurement,EDS,and drug release test,respectively.SEM and laser scanning confocal microscope(LSCM)were used to observe the growth of periodontal ligament stem cells(PDLSCs)on the 2 groups of electrospun membranes,and CCK-8 assay was used to detect the cell proliferation.Results:Both electrospun membranes had extracellular matrix(ECM)-like fiber structures.The PLGA/HMSN/Met electrospun membranes could slowly release Met for up to 35 days,and the hydrophilicity of PLGA membranes was improved by HMSNMet doped.The composite electrospun membranes had good cell biocompatibility in vitro,and could promote cell proliferation.Conclusion:Modification of PLGA with HMSN-Met can improve the hydrophilicity of PLGA electrospun membranes,continuously release Met,and have good cell biocompatibility.

关 键 词：二甲双胍 中空介孔二氧化硅 聚乳酸-羟基乙酸共聚物 

分 类 号：R781.4[医药卫生—口腔医学] R783.1[医药卫生—临床医学]