精氨酸改性Clinpro^(TM)窝沟封闭剂对其性能的影响  

The effects of arginine on the properties of Clinpro^(TM) pit and fissure sealant

作　　者：王瑜[1] 张云阳 邢向辉 WANG Yu;ZHANG Yunyang;XING Xianghui(Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Institute of Stomatology,Nanjing University,210008,China;Centre of Modern Analysis,Nanjing University)

机构地区：[1]南京大学医学院附属口腔医院,南京市口腔医院,南京大学口腔医学研究所,210008 [2]南京大学现代分析中心

出　　处：《实用口腔医学杂志》2024年第2期193-197,共5页Journal of Practical Stomatology

基　　金：2020年“江苏特聘医学专家”项目(编号:2021-39)。

摘　　要：目的:将精氨酸添加到Clinpro^(TM)窝沟封闭剂中进行改性,比较改性对窝沟封闭剂性能的影响。方法:配制出精氨酸浓度分别为3%、5%、10%的窝沟封闭剂(Arg3组、Arg5组、Arg10组),比较不同窝沟封闭剂的表面硬度、聚合转化率、界面微渗漏。扫描电镜观察固化后窝沟封闭剂的形貌。用液相色谱质谱法测定不同时间点窝沟封闭剂中精氨酸的释放情况。结果:各组窝沟封闭剂的表面硬度、聚合转化率没有显著性差异。Arg10组的界面渗漏率明显高于其他组(P<0.05),其余各组间无明显差异。扫描电镜观察精氨酸改性后的窝沟封闭剂均有不同程度的离子团聚现象。在24 h内的各个时间段Arg5组比Arg3组释放的精氨酸量要多,差异均有统计学意义(P<0.05)。结论:添加5%精氨酸不会影响Clinpro^(TM)窝沟封闭剂的性能及界面微渗漏,Arg5组具有好的精氨酸释放能力。Objective:To evaluate the effects of arginine on the mechanic properties of Clinpro^(TM)pit and fissure sealant.Methods:Experimental pit and fissure sealants were formulated with arginine at 3%,5%,and 10%(Arg3,Arg5 and Arg10)respectively added into Clinpro^(TM),the surface microhardness(SMH),degree of conversion(DC)and microleakage of the samples were investigated.The morphology of different pit and fissure sealants after curing were observed by scanning electron microscope(SEM).The concentration of arginine released at different time points were analyzed by Liquid Chromatography Mass Spectrometry(LC-MS/MS).Results:The SMH and DC was not statistically different among the groups,the microleakage level in Arg 10 group was higher than that in other groups(P<0.05),and there was no significant difference among other groups.There were some aggregation of arginine particles in modified agents under SEM.The Arg5 showed a significantly higher release rate of arginine at any time point than Arg3 in 24 hours(P<0.05).Conclusion:Incorporation of 5%arginine does not affect the physical and mechanical properties of Clinpro^(TM) pit and fissure sealant and exhibites good arginine release ability.

关 键 词：精氨酸 窝沟封闭剂 表面硬度 聚合转化 微渗漏 

分 类 号：R780.1[医药卫生—口腔医学]