高正加速度重复暴露环境对SD大鼠种植体骨结合的影响  

The effects of repeated high acceleration on implant osseointegration in SD rats

作　　者：周小妮 刘向东 纪英博 黄帅 宋应亮 马威 ZHOU Xiaoni;LIU Xiangdong;JI Yingbo;HUANG Shuai;SONG Yingliang;MA Wei(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Engineering Research Center for Dental Materials and Advanced Manufa,Department of Oral Implants,The Third Hospital Affiliated of Air Force Military Medical University,710032 Xi'an,China;Department of Stomatology,The 922th Hospital of Joint Logistics Support Force,Hengyang)

机构地区：[1]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔生物工程工程技术中心,空军军医大学第三附属医院种植科,西安710032 [2]联勤保障部队第九二二医院口腔科

出　　处：《实用口腔医学杂志》2024年第2期204-209,共6页Journal of Practical Stomatology

基　　金：空军军医大学第三附属医院新技术新业务重大项目(编号:LX2021-103)。

摘　　要：目的:以小动物离心机模拟飞行员高正加速度(+Gz)暴露环境,观察其对SD大鼠种植体骨结合的影响。方法:18只SD大鼠随机分为+Gz重复暴露组和对照组,每组9只,每只动物左右侧股骨远心端分别植入一枚纯钛种植体。术后24 h内实验组进入+Gz暴露环境,4~9 G 1 G/s逐渐增加,每周3次,对照组不作特殊处理。分别于2、4、8周随机处死两组各3只动物取材,进行micro CT扫描、双荧光标记和组织学分析,观察种植体周围骨结合情况。结果:2周时,+Gz重复暴露组骨体积分数(BV/TV)、骨小梁数量(Tb.N)、新骨沉积速率(MAR)、种植体-骨接触率(BIC)、种植体周围新骨生成量(BA)均显著低于对照组(P<0.05),骨小梁间隙(Tb.Sp)显著高于对照组(P<0.01);4周时,+Gz重复暴露组MAR、BA显著低于对照组(P<0.05);8周时两组各指标均无统计学差异。结论:种植体植入后即刻进入+Gz重复暴露环境使SD大鼠骨结合初期形成速度减缓;但随着+Gz加载时间的延长,这种不利影响逐渐消退,最终达到与对照组相似的骨结合情况。Objective:To investigate the effects of repeated high acceleration(+Gz)on implant osseointegration in SD rats.Methods:18 SD rats were divided into+Gz and control groups randomly(n=9),and 1 implant was placed in each femur of the rat's lower limb.24 hours postoperatively,the experimental rats were exposed to+Gz of 4 to 9 G with 1 G/s environment 3 times a week,while the control rats were fed normally.3 rats from each of the 2 groups were sacrificed at 2,4 and 8 weeks after implantation.Micro-CT,sequential fluorescence double labeling,and histological examination were perfomed for the analysis of implant osseointegration.Results:The bone volume fraction(BV/TV),trabecular number(Tb.N),mineral apposition rate(MAR),implant-bone contact rate(BIC)and bone area in implant thread(BA)of the+Gz group were significantly lower than those of the control group at 2 weeks(P<0.05),and so as to MAR,BA at 4 weeks(P<0.05),while there was no significant difference of the parameters at 8 weeks after implantation.Conclusion:In SD rats early exposure to+Gz environment postoperatively may have a negative effect on initial osseointegration by slowing bone formation.However,it will not lead to poorer bone mass when sustained over a long period.

关 键 词：+GZ重复暴露 种植体 骨结合 

分 类 号：R783.6[医药卫生—口腔医学]