CD19^(+)CD27^(+)IgD^(+)B细胞与甲状腺相关性眼病患者疾病活动度相关性研究  

The correlational research on CD19^(+)CD27^(+)IgD^(+)B cells and disease activity in patients with thyroid-associated ophthalmopathy

作　　者：冯月兰[1] 刘志英[1] 王媛媛[1] 张伟[1] FENG Yuelan;LIU Zhiying;WANG Yuanyuan;ZHANG Wei(First Affiliated Hospital of Baotou Medical College,Baotou 014010,China)

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010

出　　处：《包头医学院学报》2024年第4期33-37,共5页Journal of Baotou Medical College

基　　金：内蒙古自治区自然科学基金项目(2021MS08047,2021MS08051,2023LHMS08018);包头医学院科学研究基金项目(BYJJ-XG202004,BYJJ-QM 201926);包头医学院青年科技计划项目(BYJJ-QNGG2022048)。

摘　　要：目的:探讨甲状腺相关性眼病(thyroid-associated ophthalmopathy,TAO)患者外周血CD19^(+)CD27^(+)IgD^(+)B细胞比例变化与病情活动度的关系。方法:采用流式细胞术检测TAO患者和健康对照组CD19^(+)CD27^(+)IgD^(+)B细胞比例,分析TAO患者CD19^(+)CD27^(+)IgD^(+)B细胞比例与健康对照组的差异,并分析CD19^(+)CD27^(+)IgD^(+)B细胞比例与TAO疾病活动度之间的相关性。结果:TAO患者CD19^(+)CD27^(+)IgD^(+)B细胞比例明显低于健康对照(P<0.05);活动性TAO患者CD19^(+)CD27^(+)IgD^(+)B细胞比例细胞显著低于非活动性TAO患者;CD19^(+)CD27^(+)IgD^(+)B细胞比例与TAO患者CAS评分呈显著负相关(r=-0.7128,P<0.001)。结论:TAO患者CD19^(+)CD27^(+)IgD^(+)B细胞比例降低,与疾病活动度呈负相关。Objective:To investigate the relationship between the proportion of CD19^(+)CD27^(+)IgD^(+)B cells in peripheral blood and disease activity in patients with thyroid-associated ophthalmopathy(TAO).Methods:Flow cytometry was used to detect the proportion of CD19^(+)CD27^(+)IgD^(+)B cells in TAO patients and healthy control groups.The difference in the proportion of CD19^(+)CD27^(+)IgD^(+)B cells between TAO patients and healthy control groups was analyzed.The correlation between the proportion of CD19^(+)CD27^(+)IgD^(+)B cells and activity was also analyzed.Results:The proportion of CD19^(+)CD27^(+)IgD^(+)B cells in TAO patients was significantly lower than that in healthy controls(P<0.05).The proportion of CD19^(+)CD27^(+)IgD^(+)B cells in active TAO patients was significantly lower than in inactive TAO patients.The proportion of CD19^(+)CD27^(+)IgD^(+)B cells was significantly negatively correlated with the CAS score of TAO patients(r=-0.7128,P<0.001).Conclusion:The proportion of CD19^(+)CD27^(+)IgD^(+)B cells in TAO patients is decreased,which is negatively correlated with disease activity.

关 键 词：甲状腺相关性眼病 疾病活动度 CD19^(+)CD27^(+)IgD^(+)B细胞 流式细胞术 

分 类 号：R771.3[医药卫生—眼科] R581[医药卫生—临床医学]