基于RNA测序探究发热对巨噬细胞吞噬沙门菌极化状态的影响  

Exploration on the impact of fever on polarization of macrophage infected with Salmonella based on RNA-sequencing

作　　者：戴婷婷 马雪寒 詹晔斐[2] 许兆军[2] DAI Tingting;MA Xuehan;ZHAN Yefei;XU Zhaojun(Ningbo University Health Science Center Ningbo35211 Zhejiang,China)

机构地区：[1]宁波大学医学部,宁波315211 [2]宁波市第二医院

出　　处：《现代实用医学》2024年第3期288-293,共6页Modern Practical Medicine

基　　金：浙江省自然科学基金(LY19H190001);宁波市医学重点扶植学科(2022-F16)。

摘　　要：目的 基于高通量RNA测序技术探究温度对吞噬伤寒沙门菌巨噬细胞极化的影响。方法 制备37、38、39、40℃ 4种温度条件下巨噬细胞感染伤寒沙门菌模型,RNA测序分析各温度条件下感染伤寒沙门菌组和未感染伤寒沙门菌组的共同差异表达基因,对巨噬细胞M1、M2极化相关基因进行转录分析和趋势分析。结果 分析得到1 256条共同上调基因,1 231条共同下调基因,将其中与巨噬细胞M1、M2极化相关基因进行转录分析和趋势分析后,发现随温度升高(低于39℃),巨噬细胞向M1极化能力逐渐增强,温度继续升高(40℃),其向M1极化能力逐渐衰减;与此同时,随温度升高,巨噬细胞向M2极化的能力也逐渐增强。结论 适当的发热有利于巨噬细胞M1极化,而温度过高则会诱导巨噬细胞提前向易产生免疫逃逸的M2极化。Objective To investigate the impact of temperature on polarization of macrophage infected with Salmonella based on the high-through RNA-sequencing technique.Methods The model of macrophage infected with Salmonella was established under different temperature conditions including 37℃,38℃,39℃and 40℃.RNA sequencing was used to analyze the common differentially expressed genes between the Salmonella typhi infected and non Salmonella typhi infected groups under various temperature conditions.Transcription analysis and trend analysis were performed on the polarization related genes of macrophages M1 and M2.Results One thousand two hundred and fifty six up-regulated and 1231 down-regulated co-expression genes were obtained by RNA-sequencing,and the genes related to M1 or M2 polarization of macrophage were analyzed by transcription level and trend analysis;then,it was found that the increase of temperature to a degree less than 39℃promoted the macrophage polarization to M1.However,M1 polarization of macrophage attenuates from 39℃to 40℃.Meanwhile,the increased temperature enhanced the polarization of macrophage to M2 state.Conclusions Proper fever favors the polarization of macrophage to M1 state,while extreme temperature induces macrophage polarization earlier to M2 state that is prone to immune evasion.

关 键 词：RNA测序 巨噬细胞极化 沙门菌 发热 

分 类 号：R364.6[医药卫生—病理学]