LBR基因低表达与甲状腺癌的相关性研究  

The correlation between Low expression of LBR and Thyroid Cancer

作　　者：周婷婷 翁一如 叶恭杰[1] ZHOU Tingting;WENG Yin;YE Gongjie(Ningbo Medical Center Lihuili Hospital,Ningbo315040,Zhejiang,China)

机构地区：[1]宁波市医疗中心李惠利医院,宁波315040

出　　处：《现代实用医学》2024年第3期294-297,F0003,共5页Modern Practical Medicine

基　　金：宁波市医学科技计划项目(2021Y01);浙江省医药卫生科技计划项目(2023KY1043)。

摘　　要：目的探讨LBR基因低表达与甲状腺癌的相关性。方法本研究利用TCGA大数据分析,探究LBR在甲状腺癌中的表达,并且寻找在不同亚型中的表达差异;通过甲基化、免疫浸润分析、免疫荧光和免疫组化等方法探讨LBR基因低表达在甲状腺癌发生及发展中的作用。结果本研究发现LBR在甲状腺癌中低表达,且在不同亚型中存在表达差异(P<0.05);ROC曲线分析显示LBR在甲状腺癌中有良好的诊断效能;通过甲基化分析发现LBR表达与启动子区两个甲基化位点均呈负相关(均P<0.05),免疫浸润分析发现LBR表达与嗜酸性粒细胞、辅助型T细胞及中央记忆型T细胞浸润均呈正相关(均P<0.05);此外,研究发现LBR与TP53存在共表达和共定位,提示LBR的抑癌作用或与TP53有关。结论LBR基因的低表达可能会促进甲状腺癌的发生及发展。Objective To investigate the correlation between low expression of LBR gene and thyroid cancer.Methods In this study,TCGA data analysis was used to explore the expression of LBR in thyroid cancer,and to find the different expression in different subtypes.The role of LBR gene in the genesis and development of thyroid cancer was investigated by methylation,immunoinfiltration analysis,immunofluorescence and immunohistochemistry.Results LBR was down-regulated in thyroid cancer,and there were differences of expression among all subtypes(P<0.05).The ROC curve showed that LBR had a good diagnostic effect of thyroid cancer.The methylation analysis revealed that LBR expression was negatively correlated with two methylation sites in the promoter region(allP<0.05).Immunoinfiltration analysis revealed a positive correlation between LBR expression and infiltration of eosinophils,helper T cells,and central memory T cells(allP<0.05).Besides,the co-expression and localization of LBR with TP 53 were found,suggesting that the tumor suppressor effect of LBR may be regulated by TP 53 expression.Conclusions Low expression of LBR promotes the occurrence and development of thyroid cancer.

关 键 词：甲状腺癌 LBR基因 低表达 

分 类 号：R736.1[医药卫生—肿瘤]