抗体工程化微型机器人清除水中新型冠状病毒的研究  

Research on Antibody-Functionalized Microrobots for Removal of SARS-CoV-2 from Water

作　　者：石敏 陈泽[1] 潘宏[1,2] 郑明彬 梁锐晶[1,2] 蔡林涛 SHI Min;CHEN Ze;PAN Hong;ZHENG Mingbin;LIANG Ruijing;CAI Lintao(Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;University of Chinese Academy of Sciences,Beijing 100049,China;The Third People’s Hospital of Shenzhen,Shenzhen 518112,China)

机构地区：[1]中国科学院深圳先进技术研究院,深圳518055 [2]中国科学院大学,北京100049 [3]深圳市第三人民医院,深圳518112

出　　处：《集成技术》2024年第1期96-105,共10页Journal of Integration Technology

基　　金：广东省重点领域研发计划项目(2020B1111540001);国家重点研发计划项目(2021YFA0910900,2019YFE0198700);广东省自然科学基金面上项目(2022A1515011493);深圳市科技计划资助项目(JCYJ20220531095814033,JCYJ20220531095812028,JCYJ20210324115607020)。

摘　　要：由于严重急性呼吸系统综合征冠状病毒2型(简称“新型冠状病毒”)(SARS-CoV-2)可在水中保持长时间的稳定性和高度传染性,因此,清除水中的新型冠状病毒成为遏制及阻断其传播的重要途径。该研究采用“点击化学”反应将新型冠状病毒S蛋白的中和抗体P2C-1F11修饰在具有自驱动特性的枯草芽孢杆菌表面,构建了一种抗体工程化微型机器人(antibody functionalized bacteria microrobot,AB-robot)。AB-robot通过P2C-1F11对新型冠状病毒S蛋白的高效靶向作用,对水中新型冠状病毒进行捕获和清除。结果表明,AB-robot在饮用水和自来水等水介质中展示了快速的自驱动能力。以新型冠状病毒假病毒为病毒污染物模型,使用AB-robot后,水介质中病毒清除率高达95%。扫描电镜表征结果显示,AB-robot表面结合了大量的病毒颗粒,进一步表明AB-robot能高效捕获病毒。综上,AB-robot的快速运动和病毒靶向性能促进了其对新型冠状病毒假病毒的即时捕获和高效清除,对防止和阻断水中新型冠状病毒的传播具有重要应用价值。The coronavirus SARS-CoV-2 remains stable and highly infective in water for a long time,which makes the removal of virus from water an important approach to contain and block its spread.In this study,an antibody-engineered microrobot(AB-robot)was fabricated by using click chemistry to functionalize self-actuating Bacillus subtilis with neutralizing antibody P2C-1F11 against the SARS-CoV-2 S protein.The AB-robot can efficiently capture and remove SARS-CoV-2 from water by targeting SARS-CoV-2 S protein with P2C-1F11.The AB-robot displays rapid self-actuation capability in various aquatic media,including drinking water and tap water.And the virus clearance percentage of AB-robot in water was up to 95%against SARS-CoV-2 pseudovirus contaminant model.Moreover,scanning electron microscopy images show that a large number of virus particles were tethered to the surface of the AB-robot,further demonstrating the effective capture capability of the AB-robot.In summary,the significant contributions of both the P2C-1F11 for virus targeting and the fast microrobot motion for contacting the virus toward immediate capture and efficient removal of SARS-CoV-2 pseudovirus,which has potential application for preventing and blocking the spread of SARS-CoV-2 in water.

关 键 词：新型冠状病毒 微型机器人 中和抗体 枯草芽孢杆菌 病毒清除 

分 类 号：X52[环境科学与工程—环境工程] Q599[生物学—生物化学]