大麻素2型受体在加速正畸牙移动中的作用  

The role of CB2 in accelerating orthodontic tooth movement

作　　者：范登莹 翟浩嫣 刘慧娟 赵圆 李东娜 乔星[1] 康文静 朱德超 刘春艳[1] Fan Dengying;Zhai Haoyan;Liu Huijuan;Zhao Yuan;Li Dongna;Qiao Xing;Kang Wenjing;Zhu Dechao;Liu Chunyan(Dept of Orthodontic,School and Hospital of Stomatology,Hebei Medical University,Shijiazhuang 050017;Dept of Orthodontics,School and Hospital of Stomatology,Hebei Medical University,Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral Diseases,Shijiazhuang 050017)

机构地区：[1]河北医科大学口腔医(学)院正畸科,石家庄050017 [2]河北医科大学口腔医(学)院重点实验室,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,石家庄050017

出　　处：《安徽医科大学学报》2024年第2期212-218,共7页Acta Universitatis Medicinalis Anhui

基　　金：2021年政府资助临床医学优秀人才项目(编号:361029);2022年引进留学人员资助项目(编号:C20220353)。

摘　　要：目的 探讨大麻素2型受体(CB2)在正畸过程中对小鼠正畸牙移动(OTM)速率和压力侧牙周组织改建的作用。方法 筛选CB2^(-/-)和同窝WT型各30只雄性小鼠,6周龄时建立牙齿移动模型,建模时间分别为0、3、7、14、21 d(n=6)后取材,经体视显微镜测量牙移动距离;HE染色观察根压力区牙周组织变化;TRAP染色统计根压力区破骨细胞数量;免疫组织化学染色观察根压力区基质金属蛋白酶-9(MMP-9)阳性单核细胞和多核细胞的数量变化。结果 正畸牙移动距离测量结果显示,在21 d内,随着时间延长,牙齿移动距离逐渐增加,且3、7、14、21 d时CB2^(-/-)组牙齿移动距离均较WT小鼠增加;HE染色显示OTM第14天时CB2^(-/-)小鼠压力区牙周膜间隙宽度大于WT小鼠(P<0.000 1);TRAP染色显示14 d时,在第一磨牙远中根压力区硬骨板处,CB2^(-/-)小鼠的破骨细胞数量大于WT小鼠(P<0.001);MMP-9免疫组织化学染色显示OTM 14 d时,在压力区牙周膜区域,CB2^(-/-)小鼠的MMP-9(+)单核细胞和MMP-9(+)多核细胞数量均大于WT小鼠(P<0.05)。结论 CB2可加速正畸力作用下牙齿移动速率。CB2缺失加速正畸牙移动是通过加速牙移动过程中压力侧骨吸收实现的。Objective To explore the effect of cannabinoid receptor 2(CB2) on orthodontic tooth movement(OTM) rate and periodontal tissue reconstruction of pressure area in mice.Methods Thirty CB2^(-/-) male mice and thirty littermate control WT male mice were individually accepted the orthodontic appliance at their age of 6 weeks.The mice were respectively scarified at 3 days,7 days,14 days and 21 days after the operation.Then the tooth movement distance was examined through the stereomicroscope.Hematoxylin-eosin staining was performed to explore the biological responses of periodontium at the distal mesial root pressure area.Anti-tartrate acid phosphatase staining was performed to calculate the number and distribution of osteoclasts at the distal mesial root pressure area,and MMP-9 was evaluated by immunohistochemistry to examine the number of MMP-9(+) monocytes and multinucleated cells in the same district as the TRAP staining.Results Compared with those WT mice at 3,7,14 and 21 days,OTM distance showed a gradual increased tendency according with experimental time over 21 days.The widths of periodontal ligament on the pressure side were markedly greater in CB2^(-/-) mice than WT mice at 7,14 and 21 days(P<0.000 1).The numbers of TRAP positive osteoclasts were significantly greater in CB2^(-/-) mice than those in WT mice at 14 days of OTM(P<0.001).MMP-9 immunohistochemical staining showed that the number of MMP-9(+) monocytes and multinucleated cells was more in CB2^(-/-) mice than that in WT mice at 14 days of OTM(P<0.05).Conclusion The absence of CB2 accelerates orthodontic tooth movement under orthodontic force.The absence of CB2 reinforces bone resorption in orthodontic tooth movement compressive area during orthodontic tooth movement.

关 键 词：大麻素2型受体 正畸牙移动 压力区 骨改建 破骨细胞 

分 类 号：R783.5[医药卫生—口腔医学]