LINC00894通过miR-205-5p/ZEB1轴对胃癌细胞增殖和转移的影响  

Effect of LINC00894 gene on proliferation and metastasis of gastric cancer cells via miR-205-5p/ZEB1 axis

作　　者：康伟彪 周理好 余昌俊[1] 江露 陈昌裕[1] Kang Weibiao;Zhou Lihao;Yu Changjun;Jiang Lu;Chen Changyu(Dept of Gastrointestinal Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Surgery,West Anhui Health Vocational College,Lu′an 237000)

机构地区：[1]安徽医科大学第一附属医院胃肠外科,合肥230022 [2]皖西卫生职业学院外科教研室,六安237000

出　　处：《安徽医科大学学报》2024年第2期282-288,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究项目(编号:KJ2021A1364、KJ2021ZD0025)。

摘　　要：目的 探究长链非编码RNA 00894(LINC00894)基因对人胃癌细胞增殖、转移的影响,验证LINC00894、miR-205-5p、ZEB1在胃癌中的调控关系。方法 采用RT-qPCR检测LINC00894在胃癌细胞系、正常胃细胞系、临床胃癌及正常胃组织样本中的表达水平;通过随访,探究LINC00894的表达水平与胃癌患者预后的关系;构建LINC00894敲减细胞系和过表达细胞系,并采用RT-qPCR检测敲减及过表达效率;通过CCK-8、克隆形成和Transwell实验检测细胞增殖与转移能力;采用双荧光素酶报告实验、RT-qPCR实验和Western blot实验检测LINC00894、miR-205-5p和ZEB1的靶向调控关系。结果 LINC00894基因在胃癌组织或细胞中的表达量明显高于胃正常组织或细胞,且LINC00894基因高表达的胃癌患者预后更差;LINC00894基因的敲减抑制了胃癌细胞的活力、克隆形成能力、迁移能力和侵袭能力,相反,LINC00894基因的过表达得到相反地结果;LINC00894通过靶向miR-205-5p并下调其表达,从而促进ZEB1的表达。结论 LINC00894在胃癌中扮演癌基因的角色,可能通过miR-205-5p/ZEB1轴促进胃癌细胞增殖和转移。Objective To investigate the effects of long non-coding RNA 00894(LINC00894) gene on proliferation and metastasis of human gastric cancer cells,and to verify the regulatory relationship of LINC00894,miR-205-5p and ZEB1 in gastric cancer.Methods The expression level of LINC00894 in gastric cancer cell lines,normal gastric lines,clinical gastric cancer and normal gastric tissue samples were determined by RT-qPCR.Through follow-up,the relationship between the expression level of LINC00894 and the prognosis of gastric cancer patients was explored.LINC00894 knockdown cell lines and overexpression cell lines were constructed,and the knockdown and overexpression efficiency was detected by RT-qPCR.Cell proliferation and metastatic capacity were determined by CCK 8,clone formation and Transwell assays.Dual-luciferase reporter assays,RT-qPCR assays and Western blot assays were used to examine the targeted regulatory relationships of LINC00894,miR-205-5p and ZEB1.Results The expression of LINC00894 gene in gastric cancer tissues or cells was significantly higher than that in normal gastric tissues or cells,moreover,gastric cancer patients with high LINC00894 gene expression had a worse prognosis.The knockdown of LINC00894 inhibited the viability,clonogenesis,migration and invasion ability of gastric cancer cells,and conversely,the overexpression of LINC00894 obtained the opposite results.LINC00894 promoted ZEB1 expression by targeted downregulation of miR-205-5p expression.LINC00894 promoted the expression of ZEB1 by targeting miR-205-5p and down-regulating its expression.Conclusion LINC00894 serves as an oncogene in gastric cancer and may promote proliferation and metastasis of gastric cancer cells via regulating miR-205-5p/ZEB1 axis.

关 键 词：胃癌 LINC00894 miR-205-5p ZEB1 

分 类 号：R735.2[医药卫生—肿瘤]