蜜蜂资源形态测定的采样与保存方法研究  

Study on Sampling and Preservation Methods for Morphological Identification of Honeybee Resources

作　　者：庄明亮[1] 李剑飞 迟永娟[1] 葛蓬[1] 王志[1] 蒋海宾 王进州[1] 戚竞文 李志勇[1] ZHUANG Mingliang;LI Jianfei;CHI Yongjuan;GE Peng;WANG Zhi;JIANG Haibin;WANG Jinzhou;QI Jingwen;LI Zhiyong(Apiculture Science Institute of Jinlin Province,Jilin 132108,China)

机构地区：[1]吉林省养蜂科学研究所,吉林吉林132108

出　　处：《特产研究》2024年第2期22-24,29,共4页Special Wild Economic Animal and Plant Research

基　　金：国家重点研发计划项目(2022YFD1600200);吉林省科技发展计划项目(YDZJ202301ZYTS332);国家蜂产业技术体系建设项目(CARS-44-KXJ3)。

摘　　要：形态测定是蜜蜂种质资源评价必不可少的技术手段,本研究旨在优化采样与保存方法来提高蜜蜂形态测定工作的效率。通过各试验组不同采样与保存方法对比蜜蜂样品的伸吻率、组织损坏数量、色度及韧性的变化。结果表明,试验组B毙杀20 s、30s的伸吻率差异不显著(P>0.05),但均显著高于其他试验组(P<0.05);试验组D的蜜蜂组织损坏数量显著低于试验组E、F、G和对照组(P<0.05);在4℃环境中保存1~3个月对蜜蜂样本的色度和样本的韧性均没有影响。蜜蜂资源形态测定的样品建议使用2L/min流速的CO_(2)毙杀20 s,浸泡在75%酒精和0.5%甘油的保存液中,保存3个月内完成形态测定工作。In order to improve the efficiency of bee morphological identification,morphological identification is an essential technique for bee germplasm evaluation.In this study,different sampling and preservation methods were used to compare the kissing rate,the amount of tissue damage,the influence of chroma and the influence of toughness of bee samples in different experimental groups.The results showed that there was no significant difference in the kissing rate of B fumigation at 20 s and 30 s in experimental groups(P>0.05),but it was significantly higher than that in other experimental groups(P<0.05).The number of honeybee tissue damage in experimental group D was significantly lower than that in experimental group E,F,G and control group(P<0.05).Storage at 4℃for 1 to 3 months had no effect on the chroma and toughness of honeybee sampls.It is recommended to use CO_(2) at the flow rate of 2 L/min for 20 s,soak in the preservation solution of 75%alcohol and 0.5%glycerol,and complete the morphological identification within 3 months.

关 键 词：蜜蜂 采样 保存 资源鉴定 形态测定 

分 类 号：S891.2[农业科学—特种经济动物饲养]