桂林古茶树资源遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of Ancient Tea Plant Resources in Guilin

作　　者：王小云 谭少波 刘诗诗 Wang Xiao-yun;Tan Shao-bo;Liu Shi-shi(Tea Science Research Institute of Guangxi Zhuang Autonomous Region,Guilin,Guangxi 541004,China)

机构地区：[1]广西壮族自治区茶叶科学研究所,广西桂林541004

出　　处：《广西农学报》2023年第6期18-22,28,共6页Journal of Guangxi Agriculture

基　　金：桂林市技术应用与推广计划项目(20210224-4);广西科技重大专项项目(桂科AA20302018-1);桂林市创新平台和人才计划项目(20220124-16);广西特色作物试验站项目(TS202126);国家现代农业产业技术体系广西茶叶创新团队桂林综合试验站项目(nycetxgxcxtd-18-04)。

摘　　要：【目的】利用ISSR分子标记技术对桂林50份古茶树资源的遗传多样性与亲缘关系进行研究,以期全面了解其基因特征,为桂林茶树种质资源评价与鉴定、种质资源筛选、资源保护与利用等提供参考。【方法】用13条引物对桂林50份古茶树单株资源和12份良种资源的遗传多样性进行分析。【结果】13条引物扩增出223条条带,210条条带具有多态性,多态性比率为94.17%,表明桂林古茶树具有丰富的遗传多样性。【结论】桂林古茶树多集中在龙胜各族自治县—资源县—兴安县一带,存在基因交流现象,遗传距离相对短。【Objective】ISSR molecular marker technology was applied to study the genetic diversity and genetic relationship of 50 ancient tea resources in Guilin,in order to fully understand their genetic characteristics,and provide reference for the evaluation and identification of tea germplasm resources,germplasm resources screening,resource protection and utilization in Guilin.【Method】The genetic diversity of 50 ancient tea plant resources and 12 improved variety resources in Guilin was analyzed by 13 primers.【Result】Thirteen primers amplified 223 bands,210 bands were polymorphic,with a polymorphism ratio of 94.17%,indicating that Guilin ancient tea trees have rich genetic diversity.【Conclusion】Guilin ancient tree tea is mostly concentrated in Longsheng County-Ziyuan County-Xing'an County.There is a phenomenon of gene exchange and the genetic distance is relatively short.

关 键 词：桂林 古茶树 遗传多样性 ISSR分析 

分 类 号：S571.1[农业科学—茶叶生产加工]